Novel synergistic mechanism for robust expression of NR2C in cerebellar granule cells

为了确定驱动新机制的生理活性物质，我在药理学上采取了扩展的候选方法。然而，所有被选中的候选人都是否定的。为了对驱动新机制的生理活性物质进行大规模筛选鉴定，我尝试构建报告分析系统。虽然报告者结构是可行的，但由于反应体积的减少，在大量筛选的情况下难以检测到报告者信号。

To identify the physiologically active substance driving the novel mechanism, I had taken expanded candidate approaches, pharmacologically. However, all selected candidates were negative. To execute the massive screening for identification of physiologically active substance driving the novel mechanism, I had tried to construct the reporter assay system. Although a reporter construct was workable, it was difficult to detect reporter signals in the case of massive screening because of reduction of reaction volume.