Novel synergistic mechanism for robust expression of NR2C in cerebellar granule cells

小脑颗粒细胞中 NR2C 稳健表达的新协同机制

• 批准号: 22790224
• 负责人: IIJIMA Kouichirou
• 金额: $ 2.16万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790224/
• 关键词: 受容体; 細胞内シグナル伝達; 受容体・細胞内シグナル伝達

To identify the physiologically active substance driving the novel mechanism, I had taken expanded candidate approaches, pharmacologically. However, all selected candidates were negative. To execute the massive screening for identification of physiologically active substance driving the novel mechanism, I had tried to construct the reporter assay system. Although a reporter construct was workable, it was difficult to detect reporter signals in the case of massive screening because of reduction of reaction volume.

为了确定驱动这一新机制的生理活性物质，我采取了扩大候选方法，药理学。然而，所有入选的候选人都是否定的。为了进行大量的筛选以鉴定驱动新机制的生理活性物质，我尝试构建了报告检测系统。尽管报告构建是可行的，但在大规模筛选的情况下，由于反应体积的减少，很难检测到报告信号。