Detection and imaging of DNA methylation by artificial protein

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To develop a new detection method of methylated DNA, an artificial protein/peptide has been developed that bind preferentially to a methylcytosine rather than the unmethylated counterpart. Based on molecular modeling calculation of interaction between a methylcytosine and zinc finger proteins, the novel artificial peptide containing phosphotyrosine has been synthesized. Structure of the peptide and binding ability toward methylated DNA was evaluated by NMR and gel mobility shift assay, respectively. Detection of methylation in duplex DNA was examined by fluorescent anisotropy using the fluorescent-labeled artificial peptide.