Molecular dissection of sister chromatid cohesion

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Cohesin is a protein complex essential for sister chromatid cohesion. The goal of this study is to understand molecular basis behind the cohesion process. My initial approach using the cohesin complexes reconstituted from recombinant subunits fell through because of some technical reasons. Unexpectedly, I have found that balancing action between cohesin and condensin, which is required for chromosome condensation, determines shapes of metaphase chromosomes in a cell-free system from frog eggs.