Molecular dissection of sister chromatid cohesion

内聚蛋白是姐妹染色单体内聚所必需的蛋白质复合体。本研究的目的是了解内聚力过程背后的分子基础。由于一些技术原因，我最初使用重组亚基重组的内聚物复合物的方法失败了。出乎意料的是，我发现染色体凝聚所需要的凝聚蛋白和凝聚蛋白之间的平衡作用，决定了青蛙卵无细胞系统中中期染色体的形状。

Cohesin is a protein complex essential for sister chromatid cohesion. The goal of this study is to understand molecular basis behind the cohesion process. My initial approach using the cohesin complexes reconstituted from recombinant subunits fell through because of some technical reasons. Unexpectedly, I have found that balancing action between cohesin and condensin, which is required for chromosome condensation, determines shapes of metaphase chromosomes in a cell-free system from frog eggs.