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Synteny between bulb onion and bunching onion based on linkage maps.

基于连锁图谱的球茎洋葱和束洋葱之间的同线性。

基本信息

批准号：
22780009
负责人：
TSUKAZAKI Hikaru
金额：
$ 2.5万
依托单位：
National Agriculture and Food Research Organization
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780009/
关键词：
タマネギ連鎖地図ゲノム比較 QTL解析着色性 DFR ネギシンテニー

项目摘要

Linkage maps have been developed for bulb onion(Allium cepa L.) and bunching onion(A. fistulosum L.). It is considered that a high level of macrosynteny exists between these 2 species since they belong to the section Cepa and have the same chromosome number(2n=16). However, a detail comparison has not evaluated because there are few common markers onto the maps of both species. The objective of this study was to evaluate synteny at the genome-wide level between bunching onion and bulb onion. As a first step, we developed a basal linkage map in F_2 population between yellow bulb onion and red shallot. Linkage map consists of 11 linkage groups with 164 markers covering 1, 040 cM. Ten linkage groups were assigned to eight chromosomes with 65 markers. Although both SNP markers(F3' H and DFR) were located on the Chr. 7, two trait markers, Pg and Ant1, were closely linked to DFR. A QTL for bulb pigmentation was also detected on the Chr. 7.All individuals homozygous for onion allele at DFR loci showed no pigmentation in F_2 individuals. The degree of red pigmentation became significantly darkened by replacing shallot allele at DFR in both F_2 and F_<2 : 3>. Therefore, DFR is one of key genes involved in bulb color in this population.

建立了洋葱(Allium cepa L.)连锁图谱。和丛生洋葱(A.fiumulosum L.)由于它们属于CEPA组，且具有相同的染色体数目(2n=16)，因此它们之间存在较高水平的大同步性。然而，由于两个物种的地图上几乎没有共同的标记，因此还没有进行详细的比较。这项研究的目的是在全基因组水平上评估丛生洋葱和鳞茎洋葱之间的共性。作为第一步，我们在F2群体中建立了黄鳞葱和红葱之间的基本连锁图谱。连锁图由11个连锁群组成，共有164个标记，覆盖1040 cM。用65个标记将10个连锁群划分到8条染色体上。虽然两个SNP标记(F3‘H和Dfr)都位于Chr上。7、PG和ANT1两个性状标记与DFR紧密连锁。在Chr上还检测到一个控制鳞茎着色的QTL。7.在F_2群体中，所有携带洋葱等位基因纯合子的个体在DFR基因座上均未出现色素沉着现象。在F2和F2：3&gt；中，通过替换DFR上的小葱等位基因，红色素沉着程度明显变暗。因此，Dfr基因是该群体中影响鳞茎颜色的关键基因之一。