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疑似戻し交配法によるニホンナシ連鎖地図の作製とQTL遺伝子座の推定

利用拟回交法建立日本梨连锁图谱并估计QTL位点

基本信息

批准号：
12760016
负责人：
井上栄一
金额：
$ 1.34万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

本年度は,QTLに支配される形質を解析するための基礎となるニホンナシ遺伝子地図の作製を目指して,ニホンナシ品種間交雑'新高'×'筑水'のF_1集団において多型を示すAFLPおよびSSRマーカーの開発を試みた.AFLP解析では16組合せのプライマーセットについてAFLPパターンの解析を行った.その結果,両親間で多型を示したフラグメントが34種類確認された.両親間で多型を示したフラグメントのF_1集団における分離比を調査した結果,34種類のうち新高だけがヘテロで持っているフラグメントが14種類,筑水だけがヘテロで持っているフラグメントが3種類得られた.一方,新高においてホモのものが2種類,筑水においてホモのものが8種類,そして残り6種類のフラグメントはいずれの分離比にも当てはまらなかった.SSR解析では,4種類のリンゴSSRプライマー,GD12,GD15,GD96,GD100を用いてニホンナシのSSR増幅を試みた.このうちGD96とGD100の2種類では種々のPCR条件を試みたにも関わらずほとんどフラグメントが増幅されなかった.一方,GD12およびGD15を用いた場合にはSSRの遺伝子型を判別するのに十分なフラグメントデータが得られた.GD12では'新高'および'筑水'においてそれぞれ157bpまたは147bpの特異的なフラグメントが増幅され,フラグメントのF_1集団における分離はいずれもヘテロ型の分離比を示した.また,両親の遺伝子型はいずれもnullを含むと考えられた.GD15では'新高'および'筑水'においてそれぞれ139bpおよび142bpの二つののフラグメントが増幅された.これらのフラグメントもF_1集団においてヘテロ型の分離比を示した.これらの結果,リンゴで報告されているSSRプライマーがニホンナシにおいても有効であることが確認された.

This year, QTL is responsible for the analysis of the distribution system. This year, the local market is used for the purpose of monitoring, and the cross-market traffic between the products of the market is at a new high. This year, there is a multi-model demonstration of the AFLP market, the SSR market, and the AFLP market. The AFLP analysis package 16 sets the target data for the AFLP market. The results show that there are 34 types of confirmations in this category. The results of the multi-model test show that the separation and separation of the F _ 1 system has been compared. The results of the 34-year high-level test show that there are three categories of information that have been obtained in this category. On the one hand, a new level of information is available. There are eight types of information in the system, and there are eight categories in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system. On the one hand, there are eight types of information in the system, such as the first class, the third category, the second category, the third Many kinds of PCR conditions, such as GD96 and GD100 2, are required. On the one hand, GD12 said that it was very important to use the SSR system to determine the accuracy of the system. GD12 is the highest in the world. GD12 is the highest in the world. On the other hand, there is a difference between the two parts of the 157bp system in terms of the size of the 147bp system, and the performance of the F _ 1 system. The sub-type null contains the following information: GD15, new high, water, 139bp, 142bp2, 142bp2, 142bp2, 142bp2. This is the first time that the separation ratio of the F _ 1 collection has been shown. Please check the results of the report. This is the SSR report. Please confirm that you are suffering from a bad situation.