Identification of wheat gliadin genes related to allergy

为了检测小麦过敏相关蛋白的抗原抗体反应水平，制备了3种小麦过敏相关蛋白多肽的多克隆抗体。用该抗体对170个小麦品系进行了western-blot分析。此外，为了了解过敏原α/β-麦醇溶蛋白多基因的基因组结构和表达，进行了基因组分析。含有α/β-醇溶蛋白基因的基因组片段通过不等交换或跳跃式复制产生重复，而α/β-醇溶蛋白基因自身重复，没有任何重组事件。它们具有相似的顺式元件和启动子结构，其独特的基因表达机制和可能的应用提出了建议。

In order to measure the level of antigen-antibody reaction against wheat storage protein related to wheat allergy, three kinds of peptide polyclonal antibody were produced. Using the antibody, about 170 lines of wheat were screened by the western-blot analysis. Furthermore, to understand genome structure and the expression of allergen α/β-gliadin multigenes, genome analysis was conducted. Duplication of genome segments containing of α/β-gliadin genes brought about through unequal crossing-over or saltatory replication, and α/β-gliadin genes for their own were duplicated without any recombination events. They have similar cis-elements and promoter structures, the mechanisms underlying their distinct gene expression and possible applications were suggested.