Study of enzymes and factors provide direct solubilization of chitin

我们发现三种来自灰色链霉菌的几丁质结合蛋白（CBPs）促进了-和-几丁质的酶促增溶。其中一种CBP和一种几丁质酶的最佳组合在孵育24 h后产生的（GlcNAc）2的量约为未加CBP的30倍。我们证明了结晶度对-几丁质溶解作用的影响是通过使用一种市售的灰球菌酶或粘质沙雷菌的几丁质溶解体系来实现的。

We revealed three chitin binding proteins (CBPs) from Streptomyces griseus boosted enzymatic solubilization of - and -chitin. The amount of (GlcNAc)2 from the best combination with one of the CBP and a chitinase was about 30 times higher than that without the CBP after 24 h incubation.We proved crystallinity was affected to -chitin solubilization by using a commercially available enzyme from S. griceus or the chitinolytic system in Serratia marcescens.