Effect of microRNA-33b on adipogenesis regulated with SREBF1 in porcine adipocytes

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Sterol regulatory element binding proteins(SREBP) are transcription regulator for cholesterol and fatty acid homeostasis. MicroRNA-33b(miR-33b) is conserved in an intron of SREBP-1 genes of most mammalians. A computational prediction suggested that target for miR-33b includes SREBP-cleavage activating protein(SCAP). Therefore we sought to investigate the effect of miR-33b on lipogenic pathway regulated with SCAP/SREBP-1 feedback in porcine subcutaneous pre-adipocytes(PSPA) cell line. Transfection of miR-33b decreased SCAP expression at both mRNA and protein levels by 4 d post transfection. Additionally, expression of lipogenic genes and triglyceride contents were degraded in miR-33b transfected PSPA. On the other hands, miR-33b transfection obviously decreased gene expression of peroxisome proliferator-activated receptor gamma-2(PPARγ2). Our results suggested that miR-33b is associated in part with attenuation of SCAP/SREBP-1 pathway, but also affected PPARγpathway which might consequently cause downregulation of lipogenesis in porcine adipocytes.