Effect of microRNA-33b on adipogenesis regulated with SREBF1 in porcine adipocytes

microRNA-33b对SREBF1调控的猪脂肪细胞脂肪生成的影响

• 批准号: 22780256
• 负责人: TANIGUCHI Masaaki
• 金额: $ 2.58万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780256/
• 关键词: 畜産; 遺伝子; 脂質; マイクロRNA; ブタ; 脂肪合成; 遺伝子発現; 脂肪細胞株; SREBP-1; PPAR

Sterol regulatory element binding proteins(SREBP) are transcription regulator for cholesterol and fatty acid homeostasis. MicroRNA-33b(miR-33b) is conserved in an intron of SREBP-1 genes of most mammalians. A computational prediction suggested that target for miR-33b includes SREBP-cleavage activating protein(SCAP). Therefore we sought to investigate the effect of miR-33b on lipogenic pathway regulated with SCAP/SREBP-1 feedback in porcine subcutaneous pre-adipocytes(PSPA) cell line. Transfection of miR-33b decreased SCAP expression at both mRNA and protein levels by 4 d post transfection. Additionally, expression of lipogenic genes and triglyceride contents were degraded in miR-33b transfected PSPA. On the other hands, miR-33b transfection obviously decreased gene expression of peroxisome proliferator-activated receptor gamma-2(PPARγ2). Our results suggested that miR-33b is associated in part with attenuation of SCAP/SREBP-1 pathway, but also affected PPARγpathway which might consequently cause downregulation of lipogenesis in porcine adipocytes.

固醇调节元件结合蛋白（SREBP）是胆固醇和脂肪酸稳态的转录调节因子。microRNA-33 b（miR-33 b）是一种在大多数人SREBP-1基因内含子中保守的基因。计算预测表明，miR-33 b的靶点包括SREBP-裂解激活蛋白（SCAP）。因此，我们试图研究miR-33 b对猪皮下前脂肪细胞（PSPA）细胞系中由SCAP/SREBP-1反馈调节的脂肪生成途径的影响。转染miR-33 b后4 d，SCAP表达在mRNA和蛋白水平均下降。此外，在miR-33 b转染的PSPA中，脂肪生成基因的表达和甘油三酯含量降低。另一方面，miR-33 b转染明显降低了过氧化物酶体增殖物激活受体γ 2（PPARγ2）的基因表达。我们的研究结果表明，miR-33 b部分参与了SCAP/SREBP-1途径的减弱，但也影响了PPARγ途径，从而可能导致猪脂肪细胞脂肪生成的下调。

项目成果

Effect of miR-33b on porcine adipocytes differentiation and development

miR-33b对猪脂肪细胞分化发育的影响

• 发表时间: 2011
• 作者: M. Taniguchi;I;Nakajima;T. Awata;S. Mikawa
• 通讯作者: S. Mikawa

ブタ皮下脂肪細胞の分化成熟過程におけるマイクロRNAの効果

microRNA对猪皮下脂肪细胞分化成熟过程的影响

• 发表时间: 2011
• 作者: 郡司庸介;武井直樹;渡邊貴之;光藤弘俊;西村亮;山下泰尚;檜垣彰吾;鈴木稔;菱沼貢;谷口雅章,中島郁世,粟田崇,美川智
• 通讯作者: 谷口雅章,中島郁世,粟田崇,美川智