Comprehensive identification of host factors involving in ebolavirus replication

点击翻译按钮获取中文摘要

For comprehensive identification of host factors involving in replication of ebolavirus, we sought to establish a screening system for such fact factors by using genetically-engineered biologically contained ebolavirus. A human-derived cell line KBM-7, which had been thought to be the most suitable for host factor screening/identification was turned out to be resistant to ebolavirus infection, suggesting that this cell line dose not express an unknown host factor(s) required for ebolavirus infection. By contrast, a hamster-derived cell line CHO was susceptible to ebolavirus, indicating that this screening project can be carried out based on CHO cells.