Comprehensive identification of host factors involving in ebolavirus replication

全面鉴定参与埃博拉病毒复制的宿主因素

• 批准号: 22790425
• 负责人: OZAWA Makoto
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790425/
• 关键词: ウイルス; 細胞; エボラウイルス; Mutagenesis; 宿主因子; 網羅的同定; Mutagnesis

For comprehensive identification of host factors involving in replication of ebolavirus, we sought to establish a screening system for such fact factors by using genetically-engineered biologically contained ebolavirus. A human-derived cell line KBM-7, which had been thought to be the most suitable for host factor screening/identification was turned out to be resistant to ebolavirus infection, suggesting that this cell line dose not express an unknown host factor(s) required for ebolavirus infection. By contrast, a hamster-derived cell line CHO was susceptible to ebolavirus, indicating that this screening project can be carried out based on CHO cells.

为了全面鉴定参与埃博拉病毒复制的宿主因子，我们试图通过使用基因工程生物包含的埃博拉病毒来建立这些因子的筛选系统。人源细胞系KBM-7被认为是最适合宿主因子筛选/鉴定的细胞系，结果证明对埃博拉病毒感染具有抗性，表明该细胞系不表达埃博拉病毒感染所需的未知宿主因子。相比之下，仓鼠来源的细胞系CHO对埃博拉病毒敏感，表明该筛选项目可以基于CHO细胞进行。

项目成果

Subclinical Brain Injury Caused by H5N1 Influenza Virus Infection

• DOI: 10.1128/jvi.00239-11
• 发表时间: 2011-05-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Shinya, Kyoko;Makino, Akiko;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Mutations in PA, NP, and HA of a pandemic (H1N1) 2009 influenza virus contribute to its adaptation to mice.

• DOI: 10.1016/j.virusres.2011.03.022
• 发表时间: 2011-06
• 期刊: Virus research
• 影响因子: 5
• 作者: Sakabe S;Ozawa M;Takano R;Iwastuki-Horimoto K;Kawaoka Y
• 通讯作者: Kawaoka Y

A Replication-Incompetent PB2-Knockout Influenza A Virus Vaccine Vector

• DOI: 10.1128/jvi.06232-11
• 发表时间: 2012-04-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Victor, Sylvia T.;Watanabe, Shinji;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Taming influenza viruses.

• DOI: 10.1016/j.virusres.2011.09.035
• 发表时间: 2011-12
• 期刊: VIRUS RESEARCH
• 影响因子: 5
• 作者: Ozawa, Makoto;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Novel influenza virus vaccines

新型流感病毒疫苗

• 发表时间: 2011