Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection

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To generate transgenic mouse ubiquitously expressing a recombinant influenza virus-like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.