Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection

为了获得广泛表达编码GFP基因的重组流感病毒样RNA的转基因小鼠，我们构建了用于表达重组RNA的质粒。用构建的质粒预转染并随后用野生型流感病毒感染的培养细胞在病毒感染的细胞中特异性表达GFP。将该质粒的转录区片段注射到小鼠受精卵中，获得了三个生殖系对注射基因呈阳性的小鼠品系。然而，动物或其尾部衍生的原代细胞即使在感染野生型流感病毒后也不表达GFP。这些结果表明，质粒的转录区应进行修饰，以完成这项研究。

To generate transgenic mouse ubiquitously expressing a recombinant influenza virus-like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.