Analysis of a crosstalk signaling between mechanical stress and lyso-phospholipid signals in periodontal ligament

LPA受体LPA1、LPA6高表达；autotaxin （lyso-PLD）也高表达，而PA-PLA_和PS-PLA_1不表达。MS刺激人PDL细胞的autotaxin mRNA。ID1 （DNA结合抑制剂1）、EGR1（早期生长反应1）、SGK1 （g蛋白信号调节因子2）和DUSP1（双特异性磷酸酶1）在PDL细胞中上调。用^ c标记的棕榈酸和溶血磷脂酰胆碱薄层色谱法分析PDL细胞产生的LPA。6h后MS诱导LPA合成酶lyso-Phospholipase D （lyso-PLD）产生。用MS或LPA处理10 min测定ERK磷酸化。采用质谱联用PDL细胞合成LPA。

LPA1 and LPA6 of LPA receptors were highly expressed; autotaxin (lyso-PLD) was also highly expressed, however, PA-PLA_ and PS-PLA_1 were not expressed. MS stimulated autotaxin mRNA in human PDL cells. ID1 (Inhibitor of DNA binding 1), EGR1 (Early growth response 1), SGK1 (Regulator of G-protein signaling 2) and DUSP1 (Dual specificity phosphatase 1) were upregulated in PDL cells. LPA produced from PDL cells was analyzed by Thin-Layer Chromatography using ^C-labeled palmitic acid and lysophosphatidylcholine. An enzyme for LPA synthesis, lyso-Phospholipase D (lyso-PLD) was induced by MS after 6h. ERK phosphorylation was determined by MS or LPA treatment at 10 min. LPA was synthesized by PDL cell with MS.