Load frequency to temporomandibular joint brings about the osteoarthritis through the asporin expression in the articular cartilage

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In the experimental group, unilateral masseter muscles of 5-week-old rats were resected to represent the animal model of deviated mandible. Two and four weeks after muscle resection, their condyles were observed to detect whether OA-like change occurs. Micro-CTwas used for three-dimensional morphological analysis. In the experimental group, the cancellous bone volume and bone density of the condyles were decreased in the resected side. For histological examination, the specimens were embedded in paraffin and5-μm-thick serial frontal sections of the TMJ were stained with toluidine blue. As a result, the morphological changes of cartilage layers were similar to the past mandibular functional lateral shift model. Furthermore, immunohistochemical staining of asporinwhich are OA-related protein and of TGF-b1 which participates in cartilage repair were performed. We revealed that the expression of asporin in the temporomandibular joint cartilage in the OA-like animal model