Effect of Ultrasound on Viability of Chondrocyte

超声对软骨细胞活力的影响

• 批准号: 22650129
• 负责人: KUROKI Hiroshi
• 金额: $ 2.01万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650129/
• 关键词: 超音波; 軟骨細胞; 刺激; 活性; 超音波刺激; 活性化

The effect of low-intensity pulsed ultrasound(LIPUS) on mRNA expression of matrix metalloproteinase-13(MMP13) of chondrocyte was investigated using real-time PCR method. Chondrocytes were isolated from articular cartilage in rat knee joints(Wistar, 12 week-old). After the chondrocytes were cultured, culture medium was changed for serum free or serum free with IL-1 beta, 100 pg/ml or 1 ng/ml concentration. Then, LIPUS was applied at 0, 7. 5, 30, 120 mW/cm2 intensity for 20-minutes. Total RNA was extracted immediately after 1-hour incubation. LIPUS could significantly inhibit the mRNA expression of MMP13 induced by IL-1 beta at 100 pg/ml concentration in intensity-dependent manner. In conclusion, LIPUS has a possibility to inhibit mRNA expression of MMP13 in intensity-dependent manner.

采用实时荧光定量PCR方法研究低强度脉冲超声（LIPUS）对软骨细胞基质金属蛋白酶13（MMP 13）mRNA表达的影响。从大鼠膝关节（Wistar，12周龄）的关节软骨中分离软骨细胞。培养软骨细胞后，将培养基更换为无血清或含100 pg/ml或1 ng/ml浓度的IL-1 β的无血清培养基。然后，在0、7. 5、30、120 mW/cm 2强度下持续20分钟。孵育1小时后立即提取总RNA。LIPUS在100 pg/ml浓度下可显著抑制IL-1 β诱导的MMP 13 mRNA表达，且呈强度依赖性。结论：LIPUS可能以强度依赖性方式抑制MMP 13 mRNA的表达。

项目成果

低出力超音波パルスは軟骨細胞に対しIL-1βによって惹起されたMMP13 mRNA発現を強度依存的に抑制する

低功率超声脉冲以强度依赖性方式抑制软骨细胞中 IL-1β 诱导的 MMP13 mRNA 表达

• 发表时间: 2012
• 作者: 伊藤明良;黒木裕士;張項凱;秋山治彦
• 通讯作者: 秋山治彦

Low-intensitypulsed ultrasound downregulates themessenger RNA expression of the matrixmetalloproteinases on an articularcartilage explants model.

低强度脉冲超声下调关节软骨外植体模型中基质金属蛋白酶的信使 RNA 表达。

• 发表时间: 2011
• 作者: Akira Ito;Tomoki Aoyama;Hiroto Mitsui,Makoto Ishibashi;Shoki Yamaguchi;XiangKai Zhang;Hiroshi Kuroki.
• 通讯作者: Hiroshi Kuroki.