Comparative glycomics toward the analysis of molecular mechanism of tumor metastasis using saccharide primer method.

利用糖引物法分析肿瘤转移的分子机制的比较糖组学。

• 批准号: 22651083
• 负责人: SATO Toshinori
• 金额: $ 1.83万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22651083/
• 关键词: ケミカルバイオロジー; 糖鎖; 糖鎖プライマー; がん細胞; がんの転移性; 糖鎖合成遺伝子; 質量分析; 糖鎖生合成経路

The development of "comparative glycomics" to analyze the glycans in cells and to compare the glycans between the cells having different metastasis using saccharide primer method is the purpose of this study. The major products obtained by saccharide primer methods from highly metastatic human A549 lung adenocarcinoma cells were sulfonated glycans. Knockdown of the sulfotransferase genes using siRNA showed the suppression of cell motility, and it was indicated that sulfonated glycans are related with the metastasis of lung carcinoma cells. The major products obtained by saccharide primer methods from low metastatic murine FBJ-S1 osteosarcoma cells compared to highly metastatic FBJ-LL cells were gangliosides such as GD1a and glycosaminoglycans such as heparan sulfate. It was indicated that the expression of heparan sulfate suppressed the metastasis of osteosarcoma cells. In this study, it was shown that saccharide primer method was useful to analyze the glycans related with the metastasis of tumor cells. And the new molecular mechanisms for tumor metastases were found by saccharide primer method.

本研究的目的是发展比较糖组学，以分析细胞中的聚糖，并使用糖引物法比较具有不同转移的细胞之间的聚糖。通过糖引物法从高转移性人肺腺癌细胞A549中获得的主要产物是磺化聚糖。利用siRNA敲低磺基转移酶基因，显示细胞运动受到抑制，表明磺化聚糖与肺癌细胞的转移有关。与高转移性FBJ-LL细胞相比，通过糖引物方法从低转移性鼠FBJ-S1骨肉瘤细胞获得的主要产物是神经节苷脂如GD 1a和糖胺聚糖如硫酸乙酰肝素。硫酸乙酰肝素的表达抑制了骨肉瘤细胞的转移。本研究表明，糖引物法可用于分析与肿瘤细胞转移相关的糖链。糖引物法发现了肿瘤转移的新分子机制。

项目成果

糖鎖プライマー法によるがん細胞の転移に関わる糖鎖の解析

使用糖链引物法分析参与癌细胞转移的糖链

• 发表时间: 2011
• 作者: 岩田隆幸;田中克典;王毅楠・山形貞子・山形達也・佐藤智典;Tashiro Eand Imoto M;今野友輔・古市悠・佐藤智典
• 通讯作者: 今野友輔・古市悠・佐藤智典

Studies on metastasis-related molecules regulated by GD1a in murine FBJ Osteosarcoma Cells and Analysis of Glycan Structures in FBJ Cells Revealed by the Saccharide Primer Method

小鼠FBJ骨肉瘤细胞GD1a调控转移相关分子的研究及糖引物法揭示FBJ细胞聚糖结构分析

• 发表时间: 2010
• 作者: Y. Wang;T. Sato;L. Wang;S. Yamagata;T. Yamagata
• 通讯作者: T. Yamagata

糖鎖プライマー法を用いたマウス骨肉腫細胞に関わる糖鎖および糖転移酵素の解析

使用糖链引物法分析小鼠骨肉瘤细胞中涉及的糖链和糖基转移酶

• 作者: Kawakami;K.;清水哲郎;王 毅楠
• 通讯作者: 王 毅楠