Novel Onsite Transformation of Plant by Direct DNA Introduction

通过直接 DNA 导入对植物进行新型现场转化

• 批准号: 22656192
• 负责人: SHIOYA Suteaki
• 金额: $ 2.11万
• 依托单位: Sojo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22656192/
• 关键词: オンサイト形質転換; タバコ; 導管ネットワーク; 成長点

Availability of newly developed onsite transformation of mature plant by direct DNA introduction into every cell via vessel network was investigated. Firstly, transportation of a variety of materials via the vessel network was examined. Tobacco was used as a model plant in the present research. It was found that the materials in the vessel were rapidly transported independently of their molecular sizes. Introduction of a plasmid pBI121 harboring beta-glucuronidase(GUS) as a reporter gene into the tobacco for long term gave its GUS expression, suggesting the possibility of the formation of the transgenic tobacco. However, GUS expression in the control was occasionally detected, indicating that the analytic method of the GUS expression should be discussed.

研究了新近开发的通过导管网络将DNA直接导入每个细胞的成熟植物的现场转化的可用性。首先，通过船舶网络的各种材料的运输进行了研究。本研究以烟草为模式植物。结果发现，容器中的材料被迅速运输，而与它们的分子大小无关。将携带GUS报告基因的质粒pBI121导入烟草中，经长时间培养，GUS基因得到表达，表明转基因烟草的形成是可能的。但对照中偶尔检测到GUS表达，说明GUS表达的分析方法有待探讨。

项目成果

Possibility of onsite transformation of plant by direct DNA introduction via vessel network

通过容器网络直接引入 DNA 来现场转化植物的可能性

• 发表时间: 2011
• 作者: Kohei Taura;Shinjiro Yamamoto;Sachiko Oriishi;Shuhei Hayashi and Suteaki Shioya
• 通讯作者: Shuhei Hayashi and Suteaki Shioya

導管を利用する植物体への直接遺伝子導入の検討

考虑使用导管将基因直接转移到植物中

• 发表时间: 2011
• 作者: 三池美佳;田浦耕平;山本進二郎;林修平;塩谷捨明
• 通讯作者: 塩谷捨明