Novel Onsite Transformation of Plant by Direct DNA Introduction

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Availability of newly developed onsite transformation of mature plant by direct DNA introduction into every cell via vessel network was investigated. Firstly, transportation of a variety of materials via the vessel network was examined. Tobacco was used as a model plant in the present research. It was found that the materials in the vessel were rapidly transported independently of their molecular sizes. Introduction of a plasmid pBI121 harboring beta-glucuronidase(GUS) as a reporter gene into the tobacco for long term gave its GUS expression, suggesting the possibility of the formation of the transgenic tobacco. However, GUS expression in the control was occasionally detected, indicating that the analytic method of the GUS expression should be discussed.