Synthesis and Application of Binding-typed Inhibitor for Infection of Influenza Virus on a Base of New Conception

新概念流感病毒感染结合型抑制剂的合成及应用

• 批准号: 22658035
• 负责人: YAMAMOTO Kenji
• 金额: $ 2.28万
• 依托单位: Ishikawa Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658035/
• 关键词: インフルエンザウイルス; 感染阻害剤; シアロ糖鎖; アルギン酸; キトサン; インフルエンザウィルス; シアリルラクトース; MDCK細胞; エンド-M; シアロ糖鎖ポリマー; 免疫蛍光抗体法

We have succeeded in simple syntheses of efficient binding-inhibitors for influenza virus, which are composed of sialyl saccharides of glycopeptides extracted from hen egg yolk. We modified alginate and chitosan with multiple sialyl saccharides using condensation reaction and reductive amination reaction, respectively. These binding-inhibitors utilize the carbohydrate recognition of influenza virus for sialic acid, thus they can prevent the virus from infection. The resulting inhibitors showed sufficient inhibitory activity against influenza virus infection in MDCK cells. Unlike the other binding-inhibitors of influenza virus, these virus inhibitors require simple step in their syntheses.

我们成功地简单合成了流感病毒的有效结合抑制剂，它们由从鸡蛋黄中提取的糖肽的唾液酸组成。分别采用缩合反应和还原胺化反应对海藻酸钠和壳聚糖进行了多唾液酸化改性。这些结合抑制剂利用流感病毒对唾液酸的糖识别作用，从而阻止流感病毒的感染。所得抑制剂在MDCK细胞中显示出足够的抗流感病毒感染的抑制活性。与其他流感病毒结合抑制剂不同，这些病毒抑制剂的合成步骤简单。