Development of new gene transfer system for small fish

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The possibility of gene therapy for zebrafish and medaka with hydrodynamic methods was examined. A condition setup for gene transfer was performed with GFP plasmid. We found few GFP positive cells in the liver. Gene transfer efficiency was small compared with the infusion of the mouse models.We established fish live fibrosis model for evaluation of liver fibrosis improvement. We found that high fat diet and carbon tetrachloride is not suitable for liver fibrosis induction and DEN treatment for two months is suitable for liver fibrosis.