Mechanism of generation of pituitary adenomas based on theidentification of pituitary tumor stem cells

基于垂体肿瘤干细胞鉴定的垂体腺瘤发生机制

• 批准号: 22659263
• 负责人: YANO Shigetoshi
• 金额: $ 1.43万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659263/
• 关键词: 下垂体腺腫; 腫瘍幹細胞; 内視鏡手術; 下垂体ホルモン; 機能性腺腫

To investigate the presence of tumor stem cells in the pituitary adenoma, and to clarifythe relationship of tumor diversity and tumor development, we cultured pituitary tumorstem cells from surgical resected specimen. Stable sphere formation was obtained in twocases of non-functioning pituitary adenoma, one case of GH-secreting pituitary adenoma,and one case of ACTH-secreting pituitary adenoma. In the condition of differentiationmedia, sphere cells obtained from GH-secreting pituitary adenoma or ACTH-secretingpituitary adenoma were positively immunostained with anti-GH antibody or anti-ACTHantibody, respectively. When inducing differentiation of pituitary adenoma stem cellsextracted from the non functional pituitary adenoma was performed, positive PRL and GHimmunostaining became to be observed. Furthermore, the presence of Nestin-positive cellswas observed in tumor tissue. From these results, the pituitary tumor stem cells may existin the tumor tissue and have the ability to differentiate into multiple directions

为了研究肿瘤干细胞在垂体腺瘤中的存在，并阐明肿瘤多样性与肿瘤发生发展的关系，我们从手术切除的标本中培养了垂体肿瘤干细胞。2例无功能垂体腺瘤、1例分泌GH的垂体腺瘤和1例分泌ACTH的垂体腺瘤均形成稳定的球体。在分化培养条件下，分别用抗GH抗体和抗ACTH抗体对GH腺瘤和ACTH腺瘤球状细胞进行免疫组化染色。从无功能的垂体腺瘤中分离出干细胞进行诱导分化时，观察到PRL和GH免疫染色阳性。此外，在肿瘤组织中还观察到Nestin阳性细胞的存在。从这些结果来看，垂体瘤干细胞可能存在于肿瘤组织中，并具有向多个方向分化的能力。

项目成果

間脳下垂体部腫瘍の内視鏡手術-その現状と課題-

间脑垂体瘤的内镜手术-现状与挑战-

• 发表时间: 2011
• 作者: Asayama Y;et al;矢野茂敏
• 通讯作者: 矢野茂敏

Surg Neurol Int.

外科神经学国际。

• 发表时间: 2012
• 期刊: Giant tumefactive perivascular spaces that expanded and became symptomatic 14 years after initial surgery.
• 作者: Kono S;Tian F;Deguchi K;Yamashita T;and Abe K;Fujimoto K
• 通讯作者: Fujimoto K

プロラクチノーマに対する内視鏡下被膜摘出術の有用性と問題点

内镜下囊膜切除术治疗泌乳素瘤的用途和问题

• 发表时间: 2011
• 期刊: 日本内分泌学会雑誌
• 作者: 矢野茂敏;秀 拓一郎;倉津純一
• 通讯作者: 倉津純一

脳神経外科領域における内視鏡手術の最新知見

神经外科领域内窥镜手术的最新研究成果

• 发表时间: 2013
• 作者: Uejima D;et al;矢野茂敏
• 通讯作者: 矢野茂敏

内視鏡単独拡大経蝶形骨洞手術に必要な外科解剖

内镜下经蝶窦扩张手术所需的手术解剖结构

• 发表时间: 2012
• 作者: Zhang X;Deguchi K;Yamashita T;Ohta Y;Shang J;Tian F;Liu N;Panin VL;Ikeda Y;Matsuura T;Abe K;矢野茂敏
• 通讯作者: 矢野茂敏