Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus

多组学研究阐明缓步动物 Ramazzottius varieornatus 脱水的分子机制

• 批准号: 22681029
• 负责人: ARAKAWA Kazuharu
• 金额: $ 12.98万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22681029/
• 关键词: メタボローム; システム生物学; プロテオーム; 乾眠; クマムシ; クリプトビオシス; マルチオミクス; バイオインフォマティクス; トランスクリプトーム; 乾燥耐性; ヨコヅナクマムシ; Hypsibius dujardini; CE-MS; LC-MS; 極限環境

Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.

陆源迟滞可以通过一种被称为脱水生物的机制来抵御极端环境和几乎完全的干旱，但其分子机制尚未被探索。为此，我们对潜在的厌水生物模式物种变种迟滞拉马佐蒂菌进行了全面的多组学分析。为了分析活动状态和脱水状态下的代谢变化，我们利用CE-TOFMS和LC-TOFMS两种高通量质谱学系统对两种状态下的代谢物进行了测量。此外，还对磷酸蛋白质组进行了分析，以观察其代谢规律。因此，虽然基因表达谱的变化仅限于活跃状态和TUN状态之间，但该物种的新陈代谢在对干燥的响应中观察到了动态变化。代谢谱的变化表明细胞内对氧化和渗透应激的反应复杂。

项目成果

Two families of novel abundant heat-soluble proteins in an anhydrobiotic tardigrade

脱水缓步动物中两个富含新型热溶性蛋白质的家族

• 发表时间: 2012
• 作者: 堀部貴大;波多野学;石原一彰;Tanaka S
• 通讯作者: Tanaka S

生命活動とは何か ー 生命と非生命の境界線を探る

什么是生命活动？ - 探索生命与非生命的界限

• 发表时间: 2012
• 作者: 角山香織;新島聡;藪内弘昭;奥野恭史;栄田敏之;荒川和晴
• 通讯作者: 荒川和晴

Pathway projector: web-based zoomable pathway browser using KEGG atlas and Google Maps API.

• DOI: 10.1371/journal.pone.0007710
• 发表时间: 2009-11-11
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kono N;Arakawa K;Ogawa R;Kido N;Oshita K;Ikegami K;Tamaki S;Tomita M
• 通讯作者: Tomita M

G-language genome analysis environment with REST and SOAP web service interfaces

• DOI: 10.1093/nar/gkq315
• 发表时间: 2010-07-01
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Arakawa, Kazuharu;Kido, Nobuhiro;Tomita, Masaru
• 通讯作者: Tomita, Masaru

Analysis of DNA repair and protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after exposure to UVC radiation.

• DOI: 10.1371/journal.pone.0064793
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Horikawa DD;Cumbers J;Sakakibara I;Rogoff D;Leuko S;Harnoto R;Arakawa K;Katayama T;Kunieda T;Toyoda A;Fujiyama A;Rothschild LJ
• 通讯作者: Rothschild LJ