Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus

陆源迟滞可以通过一种被称为脱水生物的机制来抵御极端环境和几乎完全的干旱，但其分子机制尚未被探索。为此，我们对潜在的厌水生物模式物种变种迟滞拉马佐蒂菌进行了全面的多组学分析。为了分析活动状态和脱水状态下的代谢变化，我们利用CE-TOFMS和LC-TOFMS两种高通量质谱学系统对两种状态下的代谢物进行了测量。此外，还对磷酸蛋白质组进行了分析，以观察其代谢规律。因此，虽然基因表达谱的变化仅限于活跃状态和TUN状态之间，但该物种的新陈代谢在对干燥的响应中观察到了动态变化。代谢谱的变化表明细胞内对氧化和渗透应激的反应复杂。

Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.