Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus

多组学研究阐明缓步动物 Ramazzottius varieornatus 脱水的分子机制

• 批准号: 22681029
• 负责人: ARAKAWA Kazuharu
• 金额: $ 12.98万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22681029/
• 关键词: メタボローム; システム生物学; プロテオーム; 乾眠; クマムシ; クリプトビオシス; マルチオミクス; バイオインフォマティクス; トランスクリプトーム; 乾燥耐性; ヨコヅナクマムシ; Hypsibius dujardini; CE-MS; LC-MS; 極限環境

Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.

Limno-priveral Tardigrades可以通过一种称为赤霉病的机制来承受极端的环境，几乎完全干燥，但其分子机制尚未探索。为此，我们使用Tardigrade Ramazzottius varieornatus进行了全面的多词分析，该分析是一种潜在的疲劳性模型物种。为了分析活性和脱水状态的代谢变化，我们使用两种类型的高通量质谱（MS）系统，CE-TOFMS和LC-TOFMS在两种情况下测量了代谢组。此外，还分析了磷酸化蛋白蛋白体以观察到代谢法规。结果，虽然在活性和TUN状态之间的基因表达谱的变化受到限制，但该物种的代谢中观察到动态变化，响应于干燥。代谢谱的变化表明对氧化和渗透应激的细胞内反应复杂。

项目成果

G-language genome analysis environment with REST and SOAP web service interfaces

• DOI: 10.1093/nar/gkq315
• 发表时间: 2010-07-01
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Arakawa, Kazuharu;Kido, Nobuhiro;Tomita, Masaru
• 通讯作者: Tomita, Masaru

Two families of novel abundant heat-soluble proteins in an anhydrobiotic tardigrade

脱水缓步动物中两个富含新型热溶性蛋白质的家族

• 发表时间: 2012
• 作者: 堀部貴大;波多野学;石原一彰;Tanaka S
• 通讯作者: Tanaka S

生命活動とは何か ー 生命と非生命の境界線を探る

什么是生命活动？ - 探索生命与非生命的界限

• 发表时间: 2012
• 作者: 角山香織;新島聡;藪内弘昭;奥野恭史;栄田敏之;荒川和晴
• 通讯作者: 荒川和晴

De novo transcriptome sequencing and assembly of tardigrade species from ultra low input mRNA-Seq

从超低输入 mRNA-Seq 进行缓步动物物种的从头转录组测序和组装

• 发表时间: 2013
• 作者: Arakawa K;Tomita M
• 通讯作者: Tomita M

ヨコヅナクマムシゲノムの構造・機能アノテーションにおけるmRNA-Seqデータの活用

利用 mRNA-Seq 数据对缓步动物基因组进行结构和功能注释

• 发表时间: 2011
• 作者: Kiyonaka S;Mori Y.;荒川和晴
• 通讯作者: 荒川和晴