Development of genetic resources preservation for domestic animals using freeze-dried somatic cells

利用冻干体细胞保存家畜遗传资源的研究进展

• 批准号: 22681035
• 负责人: MATSUKAWA Kazutsugu
• 金额: $ 9.73万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22681035/
• 关键词: 遺伝子資源保全; 凍結乾燥; 牛; 核移植; 家畜; 遺伝資源保存・再生; 哺乳動物体細胞の保存; 遺伝資源保存; ウシ

Freeze-dried somatic cells have been proposed as a new tool for production of clone animals, since it can overcome disadvantages of the current cryopreservation method. Here, we investigated the DNA damage on bovine fibroblast cells after lyophilization, and the influence of storage temperature of FD cells on in vitro development of oocytes after NT. The rate of DNA-damaged cells was 22% in FD cells preserved at -30C, and 100% at +20C. The cleavage rates of NT embryos with fresh cells, FD cells stored at -30C, and those stored at +20C were 67%, 50%, and 0%, respectively. The blastocyst formation rates were 37%, 28%, and 0%, respectively. No significant differences were found between FD cells stored at -30C and fresh cells with regard to cleavage and development to the blastocyst stage. These results suggest that bovine blastocysts are successfully produced from FD cell-NT oocytes.

已经提出了冻干的体细胞作为生产克隆动物的新工具，因为它可以克服当前冷冻保存方法的缺点。在这里，我们研究了冻干后对牛成纤维细胞的DNA损伤，以及FD细胞的储存温度对NT后卵母细胞体外发育的影响。在-30℃下的FD细胞中，DNA损害细胞的速率为22％，在 +20c时为100％。 NT胚胎与新鲜细胞，储存在-30C和 +20C下的FD细胞的裂解速率分别为67％，50％和0％。胚泡的形成率分别为37％，28％和0％。在裂解和发育到胚泡阶段，未发现-30C的FD细胞与新鲜细胞之间的FD细胞之间没有显着差异。这些结果表明，牛胚泡是由FD细胞-NT卵母细胞成功产生的。

项目成果

ウシ体細胞の凍結乾燥後の保存法がDNA損傷度および核移植後の発生に及ぼす影響

牛体细胞冻干后保存方法对核移植后DNA损伤程度及发育的影响

• 发表时间: 2011
• 作者: J. Rátky;I. Egerszegi;P. Toth;S. Keonuchan;K-P. Brüssow;T. Nagai;K. Kikuchi;N. Manabe;田仲梨絵,有村隼,松川和嗣,葛西孫三郎,枝重圭祐;長谷川由貴,濱本圭佑,保地眞一,長尾さや子,枝重圭祐,葛西孫三郎,松川和嗣;長尾さや子,濱本圭佑,枝重圭祐,葛西孫三郎,保地眞一,松川和嗣
• 通讯作者: 長尾さや子,濱本圭佑,枝重圭祐,葛西孫三郎,保地眞一,松川和嗣

Influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells

属间/种间线粒体注射的影响；

• 发表时间: 2012
• 期刊: J Reprod Dev
• 作者: Takeda K;Srirattana K;Matsukawa K;Akagi S;Kaneda M;Tasai M;Nirasawa K;Pinkert CA;Parnpai R;Nagai T
• 通讯作者: Nagai T

ウシ核移植胚の集合処理によるクローン牛作出

牛核移植胚胎集体加工生产克隆牛

• 发表时间: 2011
• 作者: Maruyama;Junko;赤木悟史
• 通讯作者: 赤木悟史

Development of single blastomeres derived from two-cell embryos produced in vitro in pigs

• DOI: 10.1016/j.theriogenology.2011.01.021
• 发表时间: 2011-07-01
• 期刊: THERIOGENOLOGY
• 影响因子: 2.8
• 作者: Dang-Nguyen, T. Q.;Kaneda, M.;Nagai, T.
• 通讯作者: Nagai, T.

凍結乾燥したウシ顆粒膜細胞の核移植に由来する胚盤胞の作出

冻干牛颗粒细胞核移植产生囊胚

• 发表时间: 2011
• 作者: J. Rátky;I. Egerszegi;P. Toth;S. Keonuchan;K-P. Brüssow;T. Nagai;K. Kikuchi;N. Manabe;田仲梨絵,有村隼,松川和嗣,葛西孫三郎,枝重圭祐;長谷川由貴,濱本圭佑,保地眞一,長尾さや子,枝重圭祐,葛西孫三郎,松川和嗣
• 通讯作者: 長谷川由貴,濱本圭佑,保地眞一,長尾さや子,枝重圭祐,葛西孫三郎,松川和嗣