The influence of human gut bacteria on bioavailability of flavonoids (Consortium: Flavonoids and polyphenols)

拟议的项目旨在阐明肠道微生物群在选定的膳食黄酮转化中的作用，并研究肠道细菌如何以及在多大程度上影响它们。预计该项目将产生的数据与口服摄入类黄酮的安全性问题有关。由于无菌（GF）状态的人类受试者不可用，因此GF大鼠和与人类肠道细菌相关的大鼠（HFA大鼠）将用作模型。将代表黄酮、花青素和异戊烯化类黄酮的选定化合物饲喂给这些大鼠。随后，将采集这些动物的血液、尿液和粪便，并通过HPLC、GC和LC-MS技术分析所用物质及其降解产物。GF和HFA大鼠的比较将表明肠道细菌是否转化摄入的化合物以及转化的程度。将鉴别细菌转化的产物，并测定其在动物胃肠道中的吸收。

The proposed project is aimed to elucidate the role of the gut microbiota in the conversion of selected dietary flavonoids and to investigate how and to which extent intestinal bacteria influence their. The data expected to emerge from the project are of relevance to safety issues as related to the oral intake of flavonoids. Since human subjects with a germ-free (GF) status are not available, GF rats and rats associated with human gut bacteria (HFA rats) will serve as models. Selected compounds representing flavones, anthocyanidins and prenylated flavonoids will be fed to these rats. Subsequently, blood, urine and feces will be collected from these animals and analyzed by HPLC, GC and LC-MS techniques for the applied substances and their degradation products. The comparison of GF and HFA rats will indicate whether and to which extent intestinal bacteria transform the ingested compounds. The products of the bacterial conversions will be identified and their absorption in the gastrointestinal tract of the animals be determined.