Behavioral and functional analysis of Shn2 gene using site-and time-specific gene targeting technique

使用位点和时间特异性基因打靶技术对 Shn2 基因进行行为和功能分析

• 批准号: 22700338
• 负责人: TAKAO Keizo
• 金额: $ 2.58万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700338/
• 关键词: 網羅的行動解析; 歯状回; 統合失調症; 遺伝子改変マウス; 行動解析

Site specific Shn2 gene knockout mice can be obtained by crossing floxed Shn2 mice with mice expressing Cre recombinase site-speficically. Mice with Shn2 deletion(Shn2Δ/Δmice) were obtained by crossing Shn2 floxed mice and telencephalin Cre mice. To confirm the deletion of the exon have the effect similar to Shn2 global knockout, we assessed maturity of the dentate gyrus of Shn2 D/D mice. RT-PCR was conducted to measure the expression of genes that are indices of the maturation of the dentate gyrus using the hippocampus of the mice. Dopamine receptor 1A was increased, desmoplakin and tryptophan 2, 3-dioxygenase were decreased in Shn2Δ/Δmice. These results suggest that Shn2Δ/Δmice have immature dentate gyrus in their adult brain.

位点特异性Shn 2基因敲除小鼠可以通过将floxed Shn 2小鼠与位点特异性表达Cre重组酶的小鼠杂交来获得。通过将Shn 2 floxed小鼠与端脑蛋白Cre小鼠杂交获得具有Shn 2缺失的小鼠（Shn 2 Δ/Δ小鼠）。为了证实外显子的缺失具有类似于Shn 2全基因敲除的效果，我们评估了Shn 2 D/D小鼠齿状回的成熟度。使用小鼠的海马进行RT-PCR以测量作为齿状回成熟的指标的基因的表达。Shn 2 Δ/Δ小鼠多巴胺受体1A增加，桥粒斑蛋白和色氨酸2，3-双加氧酶减少。这些结果表明，Shn 2 Δ/Δ小鼠在其成年脑中具有未成熟的齿状回。

项目成果

Comprehensive behaviouraGluR4 knockout mice : il study of mplication in cognitive function.

GluR4 基因敲除小鼠的综合行为：认知功能的影响研究。

• 发表时间: 2010
• 期刊: Genes, Brain and Behavior
• 作者: Tanaka N;Waki K;Kaneda H;Suzuki T;Yamada I;Furuse T;Kobayashi K;Motegi H;Toki H;Inoue M;Minowa O;Noda T;Takao K;Miyakawa T;Takahashi A;Koide T;Wakana S;Masuya H;Kota Tamada;Noriyuki Sagata
• 通讯作者: Noriyuki Sagata

Immature dentate gyros as a candidate endophenotype of psychiatric disorders.

不成熟的齿状陀螺作为精神疾病的候选内表型。

• 发表时间: 2011
• 期刊: Nihon shinkei seishin yakurigaku zasshi
• 作者: Takeuchi H;Iba M;Inoue H;Higuchi M;Takao K;Tsukita K;Karatsu Y;Iwamoto Y;Miyakawa T;Suhara T;Trojanowski JQ;Lee VM;Takahashi R;Taku Kaitsuka;Ikuko Yao;Mahito Yamada;Yasunari Yamanaka;Keizo Takao
• 通讯作者: Keizo Takao

APC1638Tマウスにみられる空間記憶障害

APC1638T 小鼠出现空间记忆障碍

• 发表时间: 2010
• 作者: Takao K;Kobayashi K;Hagihara H;Ohira K;Toyama K;Shoji H;Nakamura H;Furuya S;Takagi T;Ishii S;Miyakawa T;Keizo Takao;H.Takeuchi;Ikuko Yao;Keizo Takao;Keizo Takao;Keizo Takao;尾之内高慶,高雄啓三,宮川剛,千田隆夫
• 通讯作者: 尾之内高慶,高雄啓三,宮川剛,千田隆夫

Increase in ATP release from astrocytes results in increase in the excitability of the hippocampus

星形胶质细胞释放的 ATP 增加导致海马体兴奋性增加

• 发表时间: 2010
• 作者: Tanaka K;Yamazaki Y;Lee H. U;Furuya K;Hida H;Takao K;Miyakawa T;Fujii S;Ikenaka K
• 通讯作者: Ikenaka K

Synaptic E3ligase SCRAPPER is crucial for hippocampus-dependent fear memory formation.

突触 E3ligase SCRAPPER 对于海马依赖性恐惧记忆的形成至关重要。

• 发表时间: 2011
• 作者: Yao I;Takao K;Miyakawa T;Ito S andSetou M
• 通讯作者: Ito S andSetou M