Regulatory mechanism of AMPA receptor dynamics by phospholipase A_2

浦肯野神经元中ampa型谷氨酸受体亚基GluR2的转运是调节小脑长期抑郁（LTD）的关键机制，它有助于某些形式的运动学习和协调。我们已经证明，胞质磷脂酶a_2 α的充分激活和随后花生四烯酸从膜磷脂中解放是GluR2在突触表面表达持续下降的必要条件。为了阐明磷脂酶A_2和花生四烯酸对GluR2动力学的调控机制，我们尝试通过光解笼化化合物、可视化内化GluR2运动和磷脂组成对比分析来时空控制局部游离花生四烯酸的释放。

Trafficking of AMPA-type glutamate receptor subunit GluR2 in Purkinje neurons is a key mechanism to modulate long-term depression(LTD) in the cerebellum, which contributes to certain forms of motor learning and coordination. We have shown that full activation of cytosolic phospholipase A_2αand consequent liberation of arachidonic acid from membrane phospholipids are required for the prolonged decrease in the synaptic-surface expression of GluR2.To elucidate the regulatory mechanism of GluR2 dynamics by phospholipase A_2 and arachidonic acid, we attempted spatial and temporal control of local free arachidonic acid liberation using photolysis of caged compounds, visualization of internalized GluR2 movement, and comparative analysis of phospholipid composition.