Development of the on chip microchamber array device for single cell gene analysis

为了分析单个细胞内容物或基因信息，我们制作了具有微尺度反应场的微腔阵列芯片。芯片衬底材料之一是硅，另一个是聚苯乙烯聚合物。在评估和优化芯片上PCR条件后，尝试从微室中的单个细胞扩增DNA。通过显微操作将单个细胞置于每个微室中。因此，我们可以成功地观察到荧光，这是与DNA扩增从一个单一的细胞。

For analyzing a single cell contents or gene information, we have fabricated the microchamber array chip which has microscale reaction fields. One of chip substrate material was silicon and other one was polystylen polymer. After evaluation and optimization of the on-chip PCR conditions, DNA amplification from a single cell in the microchamber, was attempted. A single cells were placed in each microchambers by micromanipulating. As a result, we could successfully observed the fluorescence which was associated with DNA amplification from a single cells.