Analyses of microbial interspecies interactions mediated by flagella

鞭毛介导的微生物种间相互作用分析

• 批准号: 23370008
• 负责人: WATANABE Kazuya
• 金额: $ 11.56万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370008/
• 关键词: 微生物生態学; 微生物ゲノム; 環境微生物; 共生; エネルギー代謝; メタゲノム; 微生物生態系

Interspecies interactions and resultant emergent functions are essential for microbial communities to play their roles in a variety of ecosystems, such as global element cycles, fermentative production, and water treatment. In modern microbiology that has developed based on pure-culture studies, however, knowledge on interspecies interactions is limited. This study was conducted to deepen our understanding of interspecies interactions, signal transduction, and emergence of community functions in methanogenic syntrophic consortia with particular focus on recently discovered flagellum-mediated interactions. We also investigated roles of flagella on the establishment of naturally occurring methanogenic communities. We found that flagellum-mediated symbiosis is important for establishing not only artificially assembled methanogenic consortia but also naturally occurring biofilm communities.

种间相互作用和由此产生的新兴功能是微生物群落在各种生态系统中发挥作用的关键，如全球元素循环，发酵生产和水处理。然而，在基于纯培养研究发展起来的现代微生物学中，对种间相互作用的了解是有限的。本研究旨在加深我们对产甲烷互养财团中物种间相互作用、信号转导和群落功能的理解，特别关注最近发现的鞭毛介导的相互作用。我们还研究了鞭毛在天然产甲烷菌群落建立中的作用。我们发现，鞭毛介导的共生是重要的，不仅建立人工组装的产甲烷财团，但也自然发生的生物膜社区。

项目成果

A method for evaluating the host range of bacteriophages using phages fluorescently labeled with 5-ethynyl-2′-deoxyuridine (EdU)

使用 5-乙炔基-2-脱氧尿苷 (EdU) 荧光标记的噬菌体评估噬菌体宿主范围的方法

• DOI: 10.1007/s00253-012-4174-1
• 发表时间: 2012
• 期刊: Applied Microbiology and Biotechnology
• 影响因子: 5
• 作者: 小原良和;高橋恒二;神納健太郎;山中一司;Yusuke Takahashi; Takato Mitsudome; Tomoo Mizugaki; Koichiro Jitsukawa; Kiyotomi Kaneda;御纏真美子，平山道世，高山大鑑，岩瀬顕秀，工藤昭彦，根岸雄一;Sayaka Ohno
• 通讯作者: Sayaka Ohno

Microbial ecology pushes frontiers in biotechnology.

微生物生态学推动了生物技术领域的前沿。

• DOI: 10.1264/jsme2.me2901rh
• 发表时间: 2014
• 期刊: Microbes and environments
• 影响因子: 2.2
• 作者: Kouzuma A;Watanabe K
• 通讯作者: Watanabe K

Physiological and transcriptomic analyses of a thermophilic

嗜热菌的生理学和转录组学分析

• 发表时间: 2014
• 期刊: aceticlastic methanogen Methanosaeta thermophila responding to ammonia stress Microbes Environ
• 作者: Kato S;Sasaki S;Watanabe K;Yumoto I;Kamagata Y
• 通讯作者: Kamagata Y

Methanogenesis facilitated by electric syntrophy via (semi)conductive iron-oxide minerals

• DOI: 10.1111/j.1462-2920.2011.02611.x
• 发表时间: 2012-07-01
• 期刊: ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 5.1
• 作者: Kato, Souichiro;Hashimoto, Kazuhito;Watanabe, Kazuya
• 通讯作者: Watanabe, Kazuya

Syntrophic interactions in biodegradative consortia in Biodegradative Bacteria.

生物降解细菌中生物降解菌群的互养相互作用。

• 发表时间: 2013
• 作者: Kouzuma A;Watanabe K
• 通讯作者: Watanabe K