Determination of optimum conditions for growing symbiotic dinoflagellates, Amphidinium species, and development of methods for its rapid mass culture

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Symbiotic dinoflagellates, Amphidinium species, are expected to be pharmaceutically useful microalgae, because they produce antitumor macrolides. A microalgae production system with a large number of cells at a high density has been developed to produce macrolide compounds efficiently. In this study, effects of culture conditions on the cellular multiplication of dinoflagellates were investigated to determine the optimum culture conditions for obtaining high yield of the microalgae. The maximum specific growth rate was obtained at a temperature of 26oC, PPFDs of0.05-0.07 mmol m-2 s-1, photoperiod of 12 h d-1, O2 concentration of 5% and CO2 concentration of 0.1%. The results demonstrate that Amphidinium species could efficiently multiply under the condition with relatively low light intensities and low O2 concentrations with additional inorganic nutrients.The optimal depth of cuilture solution was 10 mm.