Molecular mechanisms of regulatory B cell inhibition on skin immunological diseases

调节性B细胞抑制皮肤免疫疾病的分子机制

• 批准号: 23390281
• 负责人: FUJIMOTO Manabu
• 金额: $ 12.4万
• 依托单位: University of Tsukuba (2013)Kanazawa University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390281/
• 关键词: 免疫; B細胞; 皮膚免疫; アレルギー; 自己免疫疾患; 細胞; 皮膚疾患

Signal transduction mechanisms responsible for IL-10 production in regulatory B cells were analyzed. BLNK is an important adaptor protein in B cell signal transduction. B cells from BLNK-deficient mice showed diminished IL-10 production in CD1dhiCD5+ B cells. Accordingly, BLNK-deficient mice exhibited augmented contact hypersensitivity response. CD1dhiCD5+ B cells from BLNK-deficient mice showed impaired Stat3 activation, which was dependent on Btk activity. Moreover, exogenous addition of IL-10 in culture markedly increased the number of IL-10-producing B cells, suggesting the presence of positive feedback loop provided by IL-10 stimulation.

分析了调节性B细胞产生IL-10的信号转导机制。BLNK是一种重要的B细胞信号转导接头蛋白。BLNK基因缺陷小鼠的B细胞在CD1dhiCD5+B细胞中IL-10的产生减少。因此，BLNK基因缺陷小鼠表现出增强的接触超敏反应。BLNK基因缺陷小鼠的CD1dhiCD5+B细胞表现出STAT3活性受损，其激活程度依赖于BTK活性。此外，外源性IL-10显著增加产生IL-10的B细胞的数量，提示存在由IL-10刺激提供的正反馈环。

项目成果

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft-Versus-Host Disease

• DOI: 10.1038/jid.2012.226
• 发表时间: 2012-12-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Doanh Le Huu;Matsushita, Takashi;Fujimoto, Manabu
• 通讯作者: Fujimoto, Manabu

Regulatory B cells in skin disease

皮肤病中的调节性 B 细胞

• 发表时间: 2013
• 作者: Ogawa Y;Kawamura T;Matsuzawa T;Aoki R;Gee P;Yamashita A;Moriishi K;Yamasaki K;Koyanagi Y. Blauvelt A;Shimada S.;Morio T.;Fujimoto M
• 通讯作者: Fujimoto M

Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease

• DOI: 10.1182/blood-2012-11-465658
• 发表时间: 2013-04-18
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Doanh Le Huu;Matsushita, Takashi;Fujimoto, Manabu
• 通讯作者: Fujimoto, Manabu