The cell transplant system using improved TRECK mice

TRECK方法对于生成模型小鼠和获得有用的供体细胞是非常有用的。然而，由于DTR是一种生长因子，过表达会引起轻微的表型异常，因此很难使DTR在小鼠全身表达。在本研究中，为了解决这些问题，我们使用了一种改良的DT受体，降低了生长因子活性，从而产生了改良的trek小鼠。改良后的trek小鼠表型正常，DTR可在小鼠全体中表达。这一改进有望扩大trek方法的适用范围。此外，我们可以证明从该小鼠移植和接受的供体细胞可以通过DT给药再次从受体小鼠中移除，以检查移植的效果。

The TRECK method is very useful for generating model mice and obtaining useful donor cells. However, it has been difficult to make DTR expressed in whole bodies of mice because DTR is a growth factor and overexpression of it induces a slightly abnormal phenotype. In this study, to solve these problems, we generated improved TRECK mice using a modified DT receptor with reduced growth factor activity. The improved TRECK mice showed a normal phenotype and the DTR could be expressed in whole mice bodies. This improvement is expected to extend the applicable range of the TRECK method. Furthermore, we could demonstrate that transplanted and accepted donor cells from this mouse could be removed again from the recipient mouse by DT administration to examine the effect of the transplantation.