Study on selective cancer therapy using apoptosis induced by sonodynamically activated drug

点击翻译按钮获取中文摘要

Recently, we have been studying antitumor activation of porphyrin compounds by ultrasound. The present study aims to the application of sonodynamically inducted apoptosis by porphyrin compounds for cancer treatment. Cells treated with ultrasound and porphyrin sompounds such as Porfimer Sodium, ATX-70, ATX-S10, NPe6 and DCPH-Na clearly showed membrane blebbing and cell shrinkag. Also, DNA ladder formation and caspase-3 activation were pbserved in cells treated with ultrasound and porphyrin compounds but not in order treatments. In addition, the combination of porphyin compounds and ultrasound enhanced nitroxide generation measured by ESR. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. These findings suggest that ultrasonically generated reactive oxygen species such as singlet oxygen is an important mediator of sonodynamically induced apoptosis.