Research on brain injury mechanism by quantitative analysis of the tissue damage

本研究的目的是研究脑组织的微损伤，以获得更好的了解头部损伤的耐受性，在动物的头部损伤机制的冲击试验中，我们开发了新的猪头旋转冲击装置。压缩应力松弛实验在体外进行，使用猪脑组织，随后使用染色方法进行分析。同时还观察了在不同载荷作用下，脑组织中神经细胞横长与纵长损伤比的分布。这表明，在30%或更大的压缩应变下，纵横比超过2.0。研究结果表明，变形神经细胞的横向长度为纵向长度的2倍，即极限纵横比为2.0时，压缩应变为30%。动物实验研究已获得日本大学伦理委员会批准，批准号为AP11E003。

The aim of this study was to examine micro injuries of brain tissue to gain a better understanding of head injury tolerance.In the animal impact tests due to head jury mechanism, we developed new head rotational impact apparatus of porcine. Compression stress relaxation experiments were conducted in vitro, using porcine brain tissue and subsequently analysed using stain methods. The distribution of the damage ratio of the transverse length to the longitudinal length of brain nerve cells in the brain tissue under loading was also examined. This indicated that at a compression strain of 30% or greater the aspect ratio exceeded 2.0. The results of this study show that a compression strain of 30% corresponds to the threshold for the extreme aspect ratio of 2.0; where the transverse length of deformed nerve cell is two times greater than longitudinal length.Research involving animal tests was approved by the Ethics Committee of Nihon University with the approval number AP11E003.