Dynamics of organelles and its regulation by membrane trafficking in Drosophila male meiotic division

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Drosophila primary spermatocytes undergoing meiotic division I have advantages that one can easily observe intracellular structures and can examine regulatory mechanism of later stages of cell divisions. We established multi-color imaging system that makes us possible to perform a time-lapse observation of cell organelles as a progression of meiotic divisions. We carried out depletion experiments of 1030 genes that are possibly involved in membrane trafficking. Consequently, we identified COPI and COPII components required for intracellular vesicle transport as essential factors of cytokinesis in male meiosis. The COPI is required for initiation of cytokinesis by mediating transport of cleavage furrow proteins or lipids toward cleavage sites. On the other hand, the COPII is indispensable for a progression of cytokinesis through maintenance of connection between contractile ring and plasma membrane. However, larval neuroblasts neither need both coatomer complexes.