Dynamics of organelles and its regulation by membrane trafficking in Drosophila male meiotic division

果蝇雄性减数分裂中细胞器的动态及其膜运输的调节

• 批准号: 23570004
• 负责人: INOUE Yoshihiro
• 金额: $ 3.49万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570004/
• 关键词: ショウジョウバエ; 減数分裂; オルガネラ; 細胞周期; メンブレントラフィック; 細胞質分裂; 細胞分裂; COPI

Drosophila primary spermatocytes undergoing meiotic division I have advantages that one can easily observe intracellular structures and can examine regulatory mechanism of later stages of cell divisions. We established multi-color imaging system that makes us possible to perform a time-lapse observation of cell organelles as a progression of meiotic divisions. We carried out depletion experiments of 1030 genes that are possibly involved in membrane trafficking. Consequently, we identified COPI and COPII components required for intracellular vesicle transport as essential factors of cytokinesis in male meiosis. The COPI is required for initiation of cytokinesis by mediating transport of cleavage furrow proteins or lipids toward cleavage sites. On the other hand, the COPII is indispensable for a progression of cytokinesis through maintenance of connection between contractile ring and plasma membrane. However, larval neuroblasts neither need both coatomer complexes.

进行减数分裂的果蝇原代精母细胞具有便于观察胞内结构和研究细胞分裂后期调控机制的优点。我们建立了多色成像系统，使我们能够对细胞器进行减数分裂过程的延时观察。我们对1030个可能参与膜运输的基因进行了耗尽实验。因此，我们确定了COPI和COPII组分所需的细胞内囊泡运输是男性减数分裂中细胞分裂的必要因素。通过介导裂解沟蛋白或脂质向裂解位点的运输，COPI是细胞分裂起始所必需的。另一方面，通过维持收缩环与质膜之间的连接，COPII对于细胞质分裂的进展是必不可少的。然而，成神经细胞幼虫不需要这两种涂层复合物。

项目成果

Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.

• DOI: 10.1371/journal.pone.0093450
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Vo N;Taga A;Inaba Y;Yoshida H;Cotterill S;Yamaguchi M
• 通讯作者: Yamaguchi M

Effect of αB-crystallin on protein aggregation in Drosophila.

• DOI: 10.1155/2012/252049
• 发表时间: 2012
• 期刊: Journal of biomedicine & biotechnology
• 作者: Tue NT;Shimaji K;Tanaka N;Yamaguchi M
• 通讯作者: Yamaguchi M

酸化ストレスがショウジョウバエ精巣内細胞のミトコンドリアに及ぼす影響についての発生遺伝学的解析

氧化应激对果蝇睾丸细胞线粒体影响的发育遗传学分析

• 发表时间: 2011
• 作者: 松尾樹;岡沙織;緒方翼;井上喜博
• 通讯作者: 井上喜博

蛍光タンパク質を基盤とした小胞他標的化RNA可視化システムの構築

基于荧光蛋白的囊泡等靶向RNA可视化系统的构建

• 发表时间: 2012
• 作者: 越田大夢;山田百子;梅河内隆成;Krause Henry;山口政光;吉田英樹
• 通讯作者: 吉田英樹

The length of male meiotic spindle is determined by the Cell volume of mature primary spermatocytes in Drosophila species.

雄性减数分裂纺锤体的长度由果蝇物种中成熟初级精母细胞的细胞体积决定。

• 发表时间: 2012
• 作者: Kitazawa;D.;Ogata;T. and Inoue;Y. H.
• 通讯作者: Y. H.