A study on novel regulation mechanism to repress precocious meiotic entry in Drosophila male

抑制雄性果蝇减数分裂早熟的新调控机制研究

• 批准号: 15570006
• 负责人: INOUE Yoshihiro
• 金额: $ 2.37万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570006/
• 关键词: Drosophila; male; meiosis; Mitosis; Cyclin; Polo; G2; M期進行; LARP; Polo; 染色体凝縮; Aurora B; LA様タンパク

The G2 phase just prior to first meiotic division in male is called a growth phase that is an essential period to ensure gene expression required for meiotic divisions and later spermiogenesis in Drosophila. A timing to initiate the first meiotic division is therefore strictly regulated by activity of CDK1. A hypomorphic mutation at the mitotic gene, meteor (mtr) in Drosophila leads to a production of smaller cells in premeiotic cysts consisting of primary spermatocytes. We found that a chromosome condensation has precociously proceeded in the "wee" cells. Another distinctive features in M phase such as nuclear membrane-break-down and formation of spindle-like structure have also already occurred in these "wee" cells within premeiotic cysts. The precocious entry of meiotic divisions did not allow these cells to grow sufficiently. These cytological observations suggest that the mtr gene is involved in a negative regulation to repress initiation of first meiotic division until a completi … More on of the growth phase in Drosophila males. Surprisingly, these precocious meiotic entry in the mtr males occurred before a sufficient expression of both Cyclin A and Cyclin B. Immunostaining experiments to detect active form of CDK1 indicate that the active MPF has not yet accumulated in cytoplasm of the "wee" cells with precociously condensed chromatin in the mtr males. Consistently, this precocious meiotic entry in mtr was observed even in mutant background of twine gene encoding a Cdc25 orthologue exclusively active before meiotic division. In addition to the precocious meiotic entry, a failure of centrosome separation, spindle abnormality and non-disjunction was also observed during meiotic divisions in the mtr males. The mtr gene corresponds to dlarp that encode a Drosophila ortholog of the LA-related protein sharing a homologous domain with human Lupas Antigen. Our immunostaining experiments localized the Meteor protein to a peripheral region of nucleus at premeiotic stages and to the centrosomes at M phase. We speculate that the Meteor has essential roles in both meiotic entry and meiotic divisions in Drosophila males. Less

雄性果蝇第一次减数分裂前的G2期被称为生长期，是果蝇减数分裂和精子形成所需基因表达的关键时期。因此，启动第一次减数分裂的时间受到CDK1活性的严格调控。果蝇有丝分裂基因meteor（mtr）的一种亚型突变导致减数分裂前包囊中由初级精母细胞组成的较小细胞的产生。我们发现，在“小”细胞中，染色体的凝聚已经早熟地进行了。减数分裂前期的胞囊内的这些“小”细胞也已出现了M期的另一显著特征，如核膜破裂和纺锤形结构的形成。减数分裂的早熟进入不允许这些细胞充分生长。这些细胞学观察结果表明，mtr基因参与了抑制第一次减数分裂开始的负调控，直到完成减数分裂。 ...更多信息 在果蝇雄性的生长阶段。令人惊讶的是，在mtr雄性中这些早熟的减数分裂进入发生在细胞周期蛋白A和细胞周期蛋白B充分表达之前。检测CDK1活性形式的免疫染色实验表明，活性MPF尚未在MTR雄性中具有早熟浓缩染色质的“wee”细胞的细胞质中积累。因此，即使在编码Cdc25直向同源物的突变体背景下，也观察到这种在mtr中的早熟减数分裂进入。除了早熟减数分裂进入外，在mtr雄性的减数分裂过程中还观察到中心体分离失败、纺锤体异常和不分离。mtr基因对应于dlarp，其编码与人Lupas抗原共享同源结构域的LA相关蛋白的果蝇直系同源物。我们的免疫染色实验定位Meteor蛋白的周边区域的核在减数分裂前阶段和中心体在M期。我们推测流星在果蝇雄性减数分裂进入和减数分裂分裂中起着重要作用。少

项目成果

遺伝子の転写:ショウジョウバエの形質転換(名取俊二編)

基因转录：果蝇转化（名取俊二编辑）

• 发表时间: 2003
• 作者: 井上喜博;西田育巧
• 通讯作者: 西田育巧

Yamaguchi, M., Hirose, F., Inoue, Y.H., Ohno, K., Yoshida, H., et al.: "Genetic Link between p53 and Genes Required for Formation of the Zonula Adeherens Junction."Cancer Sci.. (in press). (2004)

Yamaguchi, M.、Hirose, F.、Inoue, Y.H.、Ohno, K.、Yoshida, H.等人：“p53 与形成小带连接点所需基因之间的遗传联系。”癌症科学..（

Takata, K, Inoue, Y.H., Hirose, F., Murakami, S., Shimanouchi, S., Sakimoto, I., et al.: "Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development"Cell Biology International. 27. 361-374 (2003)

Takata, K、Inoue, Y.H.、Hirose, F.、Murakami, S.、Shimanouchi, S.、Sakimoto, I.等人：“果蝇线粒体转录因子 A 在发育过程中的时空表达”国际细胞生物学。

Characterization of dRFX2, a novel RFX family protein in Drosophila

• DOI: 10.1093/nar/gkh895
• 发表时间: 2004-01-01
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Otsuki, K;Hayashi, Y;Yamaguchi, M
• 通讯作者: Yamaguchi, M

Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development.

果蝇线粒体转录因子 A 在发育过程中的时空表达。

• 发表时间: 2003
• 期刊: Cell Biology International 27
• 作者: Takata;K;Inoue;Y.H.;Hirose;F.;Murakami;S.;Shimanouchi;S.;Sakimoto;I.;Sakaguchi;K.
• 通讯作者: K.