The degradation pathway of plant N-glycans and functional analysis of the N-glycan degradation-deficient mutant

阐明了高甘露糖型N-聚糖在植物细胞中的降解途径。在这项研究中，我们确定了一个基因编码（α）1，3-岩藻糖苷酶作用于Fuc（α）1-3GlcNAc连接在植物复杂型N-聚糖。底物特异性分析揭示了植物复合型N-聚糖的降解途径。（α）1，3-岩藻糖苷酶缺陷突变体改变了植物细胞中N-聚糖的组成，表明（α）1，3-岩藻糖苷酶是植物N-聚糖降解中必需的糖苷酶。

The degradation pathway of high-mannose type N-glycans in plant cells has been clarified. In this study, we identified a gene encoding (alpha)1,3-fucosidase acting on Fuc(alpha)1-3GlcNAc linkage in plant complex type N-glycans. Its substrate specificity analysis revealed the degradation pathway of plant complex type N-glycans. The (alpha)1,3-fucosidase-deficient mutant changed the composition of N-glycans in plant cells, showing that (alpha)1,3-fucosidase is an essential glycosidase in plant N-glycan degradation.