The translation inactivation in the pseudogenization process of chloroplast genes

叶绿体基因假生成过程中的翻译失活

• 批准号: 23570276
• 负责人: NAKAMURA Masayuki
• 金额: $ 3.41万
• 依托单位: Nagoya University (2013)Nagoya City University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570276/
• 关键词: 葉緑体; 翻訳; 共生; リボソームタンパク質; in vitro翻訳系

Plants have evolved in the direction of decreasing chloroplast genes. It remains unclear the pseudogenization process of chloroplast genes. In many higher plants, the gene for chloroplast ribosomal protein S16, rps16, is encoded not only by the nuclear genome but also by the chloroplast genome. Thus, it is possible that the pseudogenization of chloroplast rps16 gene is in progress. In this study, we found that the rps16 gene in tobacco chloroplasts is well transcribed and its transcript is correctly spliced, but mRNA is not translated. Furthermore, we determined the cis-regulatory element for translation inactivation of rps16 mRNA in tobacco chloroplasts. From these results, the translation inactivation may be the one of the triggers to become the pseudogenes in the chloroplast genome.

植物已经朝着减少叶绿体基因的方向进化。叶绿体基因的假原化过程尚不清楚。在许多高等植物中，叶绿体核糖体蛋白S16的基因rps16不仅由核基因组编码，也由叶绿体基因组编码。因此，叶绿体rps16基因的假基因化可能正在进行中。在本研究中，我们发现烟草叶绿体中的rps16基因转录良好，其转录本被正确剪接，但其mRNA没有被翻译。此外，我们还确定了烟草叶绿体中rps16mRNA翻译失活的顺式调控元件。根据这些结果，翻译失活可能是叶绿体基因组中成为假基因的触发因素之一。

项目成果

The expression of the gene for chloroplast ribosomal priten S16 is translationally repressed in tobacco

烟草中叶绿体核糖体 priten S16 基因的表达受到翻译抑制

• 发表时间: 2013
• 作者: Nakamura;M. and Sugiura;M
• 通讯作者: M

Translation inhibition of rps16 mRNA in tobacco chloroplasts is caused by the 5’ untranslated region.

烟草叶绿体中 rps16 mRNA 的翻译抑制是由 5 非翻译区引起的。

• 发表时间: 2012
• 作者: Nakamura;M. and Sugiura;M.
• 通讯作者: M.

Translation inhibition of rps16 mRNA in tobacco chloroplasts is caused by the 5'untranslated region

烟草叶绿体中rps16 mRNA的翻译抑制是由5非翻译区引起的

• 发表时间: 2012
• 作者: Nakamura;M. and Sugiura;M.
• 通讯作者: M.

rps16 gene in tobacco chloroplasts is well transcribed and its transcript is correctly spliced, but mRNA is not translated

烟草叶绿体中的rps16基因转录良好，其转录物剪接正确，但mRNA不翻译

• 发表时间: 2011
• 作者: Nakamura;M. and Sugiura;M
• 通讯作者: M

タバコ葉緑体rps16 mRNAの翻訳抑制にかかわるシス配列の同定

烟草叶绿体 rps16 mRNA 翻译抑制中涉及的顺式序列的鉴定

• 发表时间: 2014
• 作者: 中邨真之;杉浦昌弘
• 通讯作者: 杉浦昌弘