Establishment of the haploid breeding method using kinetochore malfunction lines

利用着丝粒故障系单倍体育种方法的建立

• 批准号: 23580007
• 负责人: NAGAKI Kiyotaka
• 金额: $ 3.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580007/
• 关键词: 半数体; 動原体

In this study, I tried to establish the haploid breeding method using kinetochore malfunction lines. First, plants expressing GFP::CENH3 (centromere specific histone H3) were made by transformations using Arabidopsis, tobacco and tomato for the purpose. Next, RNAi constructs to knock down internal CENH3 were transformed to the plants. Some transformants having these constructs were isolated but expression levels of internal CENH3 in the transformants were as same as these in wild type. These results suggested that RNAi method is not sufficient for the purpose.

本研究试图建立利用着丝点突变系进行单倍体育种的方法。首先，表达GFP：：CENH 3（着丝粒特异性组蛋白H3）的植物通过使用拟南芥、烟草和番茄的转化来制备。接下来，将敲低内部CENH 3的RNAi构建体转化到植物中。分离了一些具有这些构建体的转化体，但转化体中内部CENH 3的表达水平与野生型中的表达水平相同。这些结果表明RNAi方法不足以达到目的。