Studies on the puberty of red seabream with special reference to gonadotropins

促卵泡激素（FSH）和黄体生成素（LH）这两种促性腺激素在鱼类的青春期中起着关键作用。我们建立了一种新的测量红鲷FSH的方法，并用该方法阐明了红鲷血浆中FSH浓度的季节变化。此外，我们通过几项体内和体外研究发现，促性腺激素释放激素刺激红鲷垂体LH分泌，但不刺激FSH分泌。此外，我们还建立了两种稳定的分别分泌红鲷FSH和LH的细胞系，并成功获得了足够量的重组激素（30 - 60 mg），可用于鱼类人工诱导性成熟的研究。

The two gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), play pivotal roles in the puberty in fish. We have newly established the method for measuring the FSH of the red seabream and elucidated the seasonal changes of FSH concentrations in the plasma of the red seabream with this method. Also, we found that gonadotropin-releasing hormone stimulates LH secretion but does not stimulate FSH secretion from the pituitary in the red seabream through the several in vivo and in vitro studies. In addition, we have established two stable cell lines that secrete FSH and LH of the red seabream, respectively and succeeded in obtaining sufficient amount of recombinant hormones (30 - 60 mg) that can be utilized for the studies for artificial induction of sexual maturation in fish.