Studies on the puberty of red seabream with special reference to gonadotropins

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The two gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), play pivotal roles in the puberty in fish. We have newly established the method for measuring the FSH of the red seabream and elucidated the seasonal changes of FSH concentrations in the plasma of the red seabream with this method. Also, we found that gonadotropin-releasing hormone stimulates LH secretion but does not stimulate FSH secretion from the pituitary in the red seabream through the several in vivo and in vitro studies. In addition, we have established two stable cell lines that secrete FSH and LH of the red seabream, respectively and succeeded in obtaining sufficient amount of recombinant hormones (30 - 60 mg) that can be utilized for the studies for artificial induction of sexual maturation in fish.