Mechanism of degradation of paralytic shellfish toxin by polyphenols

多酚类物质降解麻痹性贝类毒素的机制

• 批准号: 23580284
• 负责人: SATO Shigeru
• 金额: $ 3.41万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580284/
• 关键词: 麻痺性貝毒; ポリフェノール; 酸化還元電位; 酸化分解; 没食子酸; 分解; ネオサキシトキシン; デカルバモイルサキシトキシン; 茶カテキン; プリン

Polyphenols (PPs) such as gallic acid and its derivatives in wine and tea were revealed to decompose 11-reduced paralytic shellfish toxins (PST). It was reported that incubation of PST with strong oxidants in such as hydro peroxide in basic solution gives fluorescent purines. A same purine derivative was found in the reaction mixture of PPs and PST after mild incubation in neutral aquatic solution. Addition of PPs to neutral phosphate buffer reduced oxidation-reduction potential of the solution, and enhanced the oxidation-degradation of PST. These findings indicate that oxidized PPs react with PST, or PPs act as effective catalysts to oxidize PST by dissolved oxygen.

葡萄酒和茶叶中的多酚类物质如没食子酸及其衍生物对11-还原型麻痹性贝类毒素具有降解作用。据报道，在碱性溶液中用强氧化剂如过氧化氢孵育PST会产生荧光嘌呤。在中性水溶液中温和孵育后，在PPs和PST的反应混合物中发现了相同的嘌呤衍生物。在中性磷酸盐缓冲液中加入PPs降低了溶液的氧化还原电位，促进了PST的氧化降解。这些结果表明，氧化PP与PST反应，或PP作为有效的催化剂，通过溶解氧氧化PST。

项目成果

Quantitative ELISA Kit for Paralytic Shellfish Toxins Coupled with Sample Pretreatment

• DOI: 10.5740/jaoacint.sgesato
• 发表时间: 2014-03-01
• 期刊: JOURNAL OF AOAC INTERNATIONAL
• 影响因子: 1.6
• 作者: Sato, Shigeru;Takata, Yoshinobu;Kodama, Masaaki
• 通讯作者: Kodama, Masaaki