Functional analysis of tubulin acetylation during mouse preimplantation development

小鼠植入前发育过程中微管蛋白乙酰化的功能分析

• 批准号: 23580416
• 负责人: KISHIGAMI Satoshi
• 金额: $ 3.33万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580416/
• 关键词: 発生; アセチル化; 翻訳後修飾; マウス; 初期胚; 卵子の老化

Histone Deacetylases (HDACs) are known to play important roles during preimplantation development. However, the roles of acetylation of non-histone proteins mouse embryos still remains poorly understood. In this study, we have focused on acetylation of alpha-tubulin and HDACs in terms of oocyte quality during one-cell stage. First, we found that treatment with NAM, nicotinamide, an inhibitor for Class III HDACs inhibited cellular fragmentation and spindle elongation after postovulatory in vitro aging. Also, the alpha-tubulin increased acetylation during aging, suggesting not only histone but non-histone protein acetylation also increases with oocyte aging. Further, once an oocyte has been activated, histone and nonhistone proteins are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation. Thus, we provide the evidence that protein acetylation play important roles for oocyte quality which affect subsequent embryonic development.

众所周知，组蛋白脱乙酰酶 (HDAC) 在植入前发育过程中发挥着重要作用。然而，小鼠胚胎非组蛋白乙酰化的作用仍然知之甚少。在这项研究中，我们重点关注 α-微管蛋白和 HDAC 的乙酰化对单细胞阶段卵母细胞质量的影响。首先，我们发现用 NAM（烟酰胺）（一种 III 类 HDAC 抑制剂）治疗可抑制排卵后体外老化后的细胞破碎和纺锤体伸长。此外，α-微管蛋白在衰老过程中乙酰化程度增加，表明组蛋白和非组蛋白乙酰化也会随着卵母细胞老化而增加。此外，一旦卵母细胞被激活，组蛋白和非组蛋白就会过度乙酰化，部分原因是 HDAC 活性降低。 TSA 处理受精卵可增强其乙酰化作用。因此，我们提供的证据表明蛋白质乙酰化对卵母细胞质量起着重要作用，从而影响随后的胚胎发育。

项目成果

Roles of protein acetylation in mouse oocyte aging

蛋白质乙酰化在小鼠卵母细胞衰老中的作用

• 发表时间: 2012
• 作者: Satoshi Komoto;Kishigami S.
• 通讯作者: Kishigami S.

Similar but distinct in vitro aging phenotypes of mouse SCNT oocytes

小鼠 SCNT 卵母细胞相似但不同的体外衰老表型

• 发表时间: 2013
• 作者: Kishigami S;Lee AR;Ha LT;Hosoi Y.
• 通讯作者: Hosoi Y.

Dynamics of lysine acetylation during the one-cell stage mouse embryos after somatic-cell nuclear transfer

体细胞核移植后单细胞阶段小鼠胚胎赖氨酸乙酰化的动态

• 发表时间: 2012
• 作者: Kishigami S、Lee AR;Matsumoto K and Hosoi Y.
• 通讯作者: Matsumoto K and Hosoi Y.

Using somatic-cell nuclear transfer to study aging

利用体细胞核移植研究衰老

• DOI: 10.1007/978-1-62703-556-9_9
• 发表时间: 2013
• 期刊: Methods Mol Biol
• 作者: Kishigami S;Lee AR;Wakayama T.
• 通讯作者: Wakayama T.

Dynamics of lysine acetylation during the one-cell stage mouse embryos

单细胞阶段小鼠胚胎赖氨酸乙酰化的动态

• 发表时间: 2011
• 作者: Kishigami S;Lee AR;Matsumoto K;Saeki K and Hosoi Y.
• 通讯作者: Saeki K and Hosoi Y.