Functional analysis of tubulin acetylation during mouse preimplantation development

组蛋白脱乙酰酶(HDAC)在着床前发育过程中发挥重要作用。然而，小鼠胚胎中非组蛋白乙酰化的作用仍然知之甚少。在这项研究中，我们主要关注α-微管蛋白和HDACs在单细胞期的乙酰化对卵母细胞质量的影响。首先，我们发现NAM，烟酰胺，一种III类HDAC的抑制剂，在体外老化后抑制了细胞的碎裂和纺锤体的延长。此外，α-微管蛋白在老化过程中增加了乙酰化，这表明不仅组蛋白，而且非组蛋白的乙酰化也随着卵母细胞的老化而增加。此外，一旦卵母细胞被激活，组蛋白和非组蛋白被过度乙酰化，部分原因是HDAC活性降低。TSA处理受精卵可增强其乙酰化作用。因此，我们提供了蛋白质乙酰化对影响后续胚胎发育的卵母细胞质量起重要作用的证据。

Histone Deacetylases (HDACs) are known to play important roles during preimplantation development. However, the roles of acetylation of non-histone proteins mouse embryos still remains poorly understood. In this study, we have focused on acetylation of alpha-tubulin and HDACs in terms of oocyte quality during one-cell stage. First, we found that treatment with NAM, nicotinamide, an inhibitor for Class III HDACs inhibited cellular fragmentation and spindle elongation after postovulatory in vitro aging. Also, the alpha-tubulin increased acetylation during aging, suggesting not only histone but non-histone protein acetylation also increases with oocyte aging. Further, once an oocyte has been activated, histone and nonhistone proteins are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation. Thus, we provide the evidence that protein acetylation play important roles for oocyte quality which affect subsequent embryonic development.