Elucidation of the molecular mechanism underlying the enhancement of somatic-cell reprogramming by a histone deacetylase inhibitor, and its applications

阐明组蛋白脱乙酰酶抑制剂增强体细胞重编程的分子机制及其应用

• 批准号: 19380161
• 负责人: KISHIGAMI Satoshi
• 金额: $ 12.9万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380161/
• 关键词: 発生工学; 核の初期化; 核移植; リプログラミング; クローン動物; ヒストン脱アセチル化酵素; マウス; 再生医療; ピストン脱アセチル化酵素

Somatic-cell nuclear transfer (SCNT) has been extremely inefficient with rates less than 5 percentage points since the first clone, "Dolly" the sheep. The representative researcher for this grant found that treatment of cloned embryos with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), dramatically increase the production rate of cloned mice more than six-times. Supported by this grant, the importance of HDAC in embryonic development and a mechanism underlying improvement of SCNT by HDACi treatment were revealed and, further cloning of "unclonable" mouse strains were achieved for the first time.

体细胞核移植（SCNT）效率极低，自第一只克隆羊“多莉”诞生以来，移植率不到5个百分点。该基金的代表性研究人员发现，用组蛋白去乙酰化酶抑制剂（HDACi）-在该基金的支持下，HDAC在胚胎发育中的重要性和HDACi治疗改善SCNT的潜在机制被揭示出来，并首次实现了“不可克隆”小鼠品系的进一步克隆。

项目成果

The impact of EGTA as chelating calcium on oocyte activation in mice

EGTA 作为螯合钙对小鼠卵母细胞活化的影响

• 发表时间: 2008
• 作者: Miyamoto A;Shirasuna K;Sasahara K;Tsuiimoto Y
• 通讯作者: Tsuiimoto Y

再生医療 8巻3号 pp. 270-273

再生医学第 8 卷第 3 页 270-273

• 发表时间: 2008
• 作者: 小林明由未;笹原希笑実;白砂孔明;赤刎幸人;松井基純;清水隆;宮本明夫;岸上哲士
• 通讯作者: 岸上哲士

Progress in DNA Methylation Research

DNA甲基化研究进展

• 发表时间: 2007
• 作者: 中江航;笹原希笑美;入江鮎美;白砂孔明;松井基純;大谷昌之;宮本明夫;Kishigami S.
• 通讯作者: Kishigami S.

Efficient strontium-induced activation of mouse oocytes in standard cuiture media by chelating calcium

通过螯合钙在标准培养介质中有效锶诱导小鼠卵母细胞激活

• 发表时间: 2007
• 期刊: J.Reprod.Dev 53
• 作者: Kawashima C;Kida K;Schweigert FJ;Miyamoto A.;Kishigami S.
• 通讯作者: Kishigami S.

Effects of trichostatin A on DNA methylation in cloned bovine embryos.

曲古抑菌素 A 对克隆牛胚胎 DNA 甲基化的影响。

• 发表时间: 2008
• 作者: Iwamoto D;Kishigami S;Taniguchi S;Abe Y;Matsui T;Kasamatsu A;Tatemizo A;Mitani T;Kato H;Matsumoto K;Hosoi Y;Wakayama T;Iritani A;Saeki K
• 通讯作者: Saeki K