Detection and quantity of vascular bundles in oil palm trunk using cell wall birefringence on exposure to polarized light

利用偏振光下细胞壁双折射检测油棕树干中维管束的数量

• 批准号: 23580463
• 负责人: MURATA Yoshinori
• 金额: $ 3.41万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580463/
• 关键词: オイルパーム; 維管束組織; 柔組織; 偏光; 複屈折; αセルロース; 維管束; トランク(幹）; セルロース; Ｘ線回析; 近赤外線; 維管束（VB）; 柔組織（PT); トランク（幹）; Elaeis guineensis; オイルパームトランク

Vascular bundles (VBs) of oil palm trunks exhibited birefringence due to polarized orientation of their cell wall components, although another component of the trunks, parenchyma tissue (PT), did not. The intensity of the birefringence depended on the VB composition; therefore, it was possible to estimate the VB content readily using a polarizing microscope connected to an image analyzer. The method proposed in this manuscript should allow easier quantification of VBs in oil palm trunks than methods using X-ray diffraction. Both VBs and PT contained cellulose, hemicelluloses, lignin, and starch; however, the cellulose content of PT was less than half that of VBs. This difference in cellulose content between VB and PT may explain why VBs were detected under polarizing light.

油棕榈树干的维管束（VB）表现出双折射，由于他们的细胞壁组件的偏振取向，虽然另一个组成部分的树干，薄壁组织（PT），没有。双折射的强度取决于VB组合物;因此，可以使用连接到图像分析仪的偏光显微镜容易地估计VB含量。在这篇手稿中提出的方法应该允许更容易量化的维生素Bs在油棕树干比使用X射线衍射的方法。VBs和PT都含有纤维素、半纤维素、木质素和淀粉，然而，PT的纤维素含量不到VBs的一半。VB和PT之间纤维素含量的这种差异可以解释为什么在偏振光下检测到VB。

项目成果

Estimation of the Ratio of Vascular Bundles to Parenchyma Tissue in Oil Palm Trunks using NIR Spectroscopy

使用近红外光谱估计油棕树干中维管束与薄壁组织的比率

• 发表时间: 2013
• 期刊: Bioresources
• 影响因子: 1.5
• 作者: Abe H;Murata Y;(他5名)
• 通讯作者: (他5名)

Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk

• DOI: 10.1016/j.biortech.2012.08.136
• 发表时间: 2012-12-01
• 期刊: BIORESOURCE TECHNOLOGY
• 影响因子: 11.4
• 作者: Prawitwong, Panida;Kosugi, Akihiko;Mori, Yutaka
• 通讯作者: Mori, Yutaka