Investigation of index of therapeutic modes as cell drugs in human mesenchymal stem cells and their validation

人间充质干细胞细胞药物治疗模式指标研究及验证

• 批准号: 23590203
• 负责人: SATOH Mitsutoshi
• 金额: $ 3.49万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590203/
• 关键词: オーダーメード医療; ヒト間葉系幹細胞; 虚血; サイトカイン; 細胞医薬品; 再生医療; 間葉系幹細胞; 免疫抑制効果; 混合リンパ球反応; 細胞増殖作用; 血管新生; 血管内皮細胞増殖因子; 低酸素誘導因子; リンパ球混合培養試験; 免疫調節; RT-PCR

We investigated the candidate genes which can become a marker to predict vascular endothelium growth factor (VEGF) secretional level of human mesenchymal stem cells (hMSCs)under ischemia. hMSCs were cultured under normal condition or ischemic condition. Cell supernatant was collected after 24hr and VEGF were measured with ELISA. We analyzed the correlation between gene expression before ischemia (Affymetrix HG-U133Plus2.0) and VEGF secretion after ischemia in hMSCs among six lots. 17 genes showed positive correlation in all combination of pre-ischemic gene expression and post-ischemic VEGF level (the change of amount or rate). Furthermore, RNAi against 2 of the 17 genes reduced VEGF secretion in response to ischemia. In this study, 17 genes were identified as candidate genes which could assess quality and efficacy of hMSCs after ischemia. In addition, it is suggested that 2 genes, the knockdown of which decrease the VEGF response, contribute to enhance VEGF secretion under ischemia.

研究了可作为预测人间充质干细胞（hMSCs）缺血时血管内皮生长因子（VEGF）分泌水平标记的候选基因。分别在正常和缺血条件下培养hMSCs。24hr后收集细胞上清，ELISA检测VEGF。我们分析6批hMSCs缺血前基因表达（Affymetrix HG-U133Plus2.0）与缺血后VEGF分泌的相关性。17个基因在缺血前基因表达与缺血后VEGF水平（变化量或变化率）均呈正相关。此外，针对17个基因中的2个的RNAi降低了缺血时VEGF的分泌。本研究确定了17个基因作为候选基因，可以评价造血干细胞缺血后的质量和疗效。此外，我们认为在缺血条件下，2个基因的敲低降低了VEGF的反应，从而促进了VEGF的分泌。

项目成果

Assessment of combined therapy with purine derivatives and infliximab in patients with refractory Crohn's disease

嘌呤衍生物与英夫利昔单抗联合治疗难治性克罗恩病的评估

• 发表时间: 2012
• 期刊: Pharmacometrics
• 作者: M. Satoh;M. Tsuchiya;Y. Takiyo;H. Doi;T. Masaka and Y. Suzuki
• 通讯作者: T. Masaka and Y. Suzuki

Correlation Between Cyclosporin A Concentration and Lipid Concentration in Patients with Ulcerative Colitis.

溃疡性结肠炎患者环孢素 A 浓度与脂质浓度之间的相关性。

• 发表时间: 2011
• 期刊: Pharmacometrics
• 作者: M. Satoh;M. Yamazaki;H. Doi;T. Masaka and Y. Suzuki
• 通讯作者: T. Masaka and Y. Suzuki

Study of immunomodulatory effects in human mesenchymal stem cells

人间充质干细胞的免疫调节作用研究

• 发表时间: 2014
• 作者: M. Satoh;T. Ohki;T. Kubo;Y. Sato and S. Muto
• 通讯作者: Y. Sato and S. Muto

Correlation between cyclosporin A concentration and lipid concentration in patients with ulcerative colitis

溃疡性结肠炎患者环孢素A浓度与血脂浓度的相关性

• 发表时间: 2011
• 期刊: Pharmacometrics
• 作者: M. Satoh;M. Yamazaki;H. Doi;T. Masaka and Y. Suzuki
• 通讯作者: T. Masaka and Y. Suzuki

ヒト脂肪由来幹細胞における虚血応答性制御因子探索研究

探索人类脂肪干细胞缺血反应调节因子的研究

• 发表时间: 2013
• 作者: Susumu Yamato;Saori Nakagawa;Yuko Kojima;Koichi Sekino;佐藤光利
• 通讯作者: 佐藤光利