Identification of the novel genes associated with vascular morphogenesis

点击翻译按钮获取中文摘要

Vascular formation progressed with a regular time course to form a uniform structure. However the associated genes of vascular morphogenesis are still poorly uncovered. To address this issue, we performed microarray analysis of etsrp/etv2 deficient zebrafish embryos. As a result, we identified 165 genes that were up-regulated greater than 1.5 fold by silencing etsrp/etv2 expression. Furthermore, we analyzed their expression patterns to evaluate whether these genes were associated with the cranial and/or truncal vascular formation by in silico analysis using ZFIN database, and selected 11 genes which were expressed in the specific region associated with the vascular formation. We also performed in situ hybridization for 68 selected genes which precise expression patterns were not obtained, and succeeded to demonstrate three new expression patterns. We are now planning to knock out these genes using CRISPR/Cas9 system to analyze their function for the vascular formation.