Sugar modification system that inhibits regeneration of injured CNS neurons

抑制受损中枢神经元再生的糖修饰系统

• 批准号: 23590221
• 负责人: WANAKA Akio
• 金额: $ 3.49万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590221/
• 关键词: OASIS; コンドロイチン硫酸; 神経再生; アストロサイト; 転写調節; ノックアウトマウス; 脳損傷; 糖鎖修飾; C6ST-1; 転写因子

We found that chondroitin sulfate proteoglycan expression is reduced in the OASIS-kockout mouse as compared to the wild type mouse. We screened that target genes of OASIS transcription in the CSPG production pathway and found that C6ST-1, an enzyme that transfers sulfate moieties to CSPG, was a potential candidate. By artificial transcription assay system, we examined whether C6ST-1 transcription is regulated by OASIS or not. The first intron of the C6ST-1 genome contained several CRE (OASIS binding site) and deletion of those CREs reduced C6ST-1 transcription. Taken together, we concluded that OASIS inhibits neuronal regeneration by regulating C6ST-1 activities in the injured central nervous system.

我们发现，与野生型小鼠相比，OASIS-kockout小鼠中硫酸软骨素蛋白聚糖的表达减少。我们筛选了CSPG产生途径中OASIS转录的靶基因，发现C6 ST-1，一种将硫酸基团转移到CSPG的酶，是一个潜在的候选者。通过人工转录检测系统，我们检测了C6 ST-1的转录是否受OASIS的调控。C6 ST-1基因组的第一个内含子含有几个CRE（OASIS结合位点），这些克雷斯的缺失降低了C6 ST-1的转录。综上所述，我们得出结论，OASIS通过调节受损中枢神经系统中的C6 ST-1活性来抑制神经元再生。

项目成果

TenascinR constitutes a unique extracellular matrix and modulates GLAST expression in astrocytes.

TenascinR 构成独特的细胞外基质并调节星形胶质细胞中的 GLAST 表达。

• 发表时间: 2012
• 作者: Okuda H;Tatsumi K;Shibukawa Y;Korekane H;Horii-Hayashi N;Wada Y;Taniguchi N;Wanaka A
• 通讯作者: Wanaka A

Chondroitin sulfate proteoglycan tenascin-R regulates glutamate uptake by adult brain astrocytes

硫酸软骨素蛋白多糖腱蛋白-R 调节成年脑星形胶质细胞谷氨酸的摄取

• DOI: 10.1074/jbc.m113.504787
• 发表时间: 2014
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Okuda H;Tatsumi K;Morita S;Shibukawa Y;Korekane H;Horii-Hayashi N;Wada Y;Taniguchi N;Wanaka A.
• 通讯作者: Wanaka A.

Inhibition of Wnt/beta-catenin pathway by Dickkopf-1 affects midfacial morphogenesis in chick embryo

Dickkopf-1 抑制 Wnt/β-catenin 通路影响鸡胚中面部形态发生

• 发表时间: 2013
• 期刊: J. Biosci. Bioeng
• 作者: Kawakami M;Okuda H;Tatsumi K;Kirita T;Wanaka A
• 通讯作者: Wanaka A

Astrocytic TRPV1 ion channels detect blood-borne signals in the se sory circumventricular organs of adult mouse brains

星形胶质细胞 TRPV1 离子通道检测成年小鼠大脑室周器官中的血源信号

• 发表时间: 2013
• 期刊: Glia
• 影响因子: 6.2
• 作者: Mannari T;Morita S;Furube E;Tominaga M;Miyata S
• 通讯作者: Miyata S

Induction of inner ear hair cell-like cells from Math1-transfected mouse ES cells.

• DOI: 10.1038/cddis.2013.230
• 发表时间: 2013-07-04
• 期刊: Cell death & disease
• 影响因子: 9