Role of associative fiber tracts on sulcal development of the cerebrum in ferrets

将出生后4 ~ 90天的雄性和雌性雪貂灌注4%多聚甲醛溶液，并去脑。脑沟和脑回突现的年表通过大体解剖观察得到了澄清。使用高空间分辨率的7特斯拉MRI进一步获取固定脑的T1W-MRI。基于t1w - mri的最大强度投影（MIP）可以三维描绘与骨髓结构相关的皮质功能区域。脑沟内折的定量分析显示，在大脑的颞顶枕区，新皮质沟和主皮质沟的后部延伸明显，皮质扩张。应用弥散张量成像技术（DTI）测量了雪貂大脑沟区和回区皮层下白质的各向异性分数。然而，脑沟发育与皮层下白质束发育的关系尚不清楚。

Male and female ferrets at postnatal days (PDs) 4 to 90 were perfused with 4% paraformaldehyde solution, and the brains were removed. The chronology of sulcal and gyral emergences were clarified using those brain samples by gross anatomic observations. T1W-MRI was further acquired from the fixed brain using a high spatial resolution 7-tesla MRI. T1W-MRI-based maximum intensity projection (MIP) could delineate functional cortical areas related to myeloarchitecure in 3D. Quantitative analysis of the sulcal infolding revealed a male-prominent posterior extension of neocortical and archicortical sulci with cortical expansion in the temporo-parieto-occipital region of the cerebrum. Fractional anisotropy was measured in the subcortical white matter of the sulcal and gyral regions of the ferret cerebra using diffusion tensor imaging (DTI). However, it was unclear a relation of sulcal development with the development of subcortical white matter bundles.