Telomere length and carcinogenesis of human bronchial epithelium, study using tissue quantitative fluorescence in situ hybridization

人支气管上皮的端粒长度和致癌作用，利用组织定量荧光原位杂交进行研究

• 批准号: 23590411
• 负责人: AIDA Shinsuke
• 金额: $ 3.41万
• 依托单位: International University of Health and Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590411/
• 关键词: テロメア; 気管支上皮; 喫煙; 肺癌; Q-FISH; FISH; 前癌病変; p53; Ki67

Telomere lengths of the bronchial epithelium of 72 non-lung cancer adult autopsy cases and 24 surgically resected cases of lung squamous cell carcinoma were measured using Q-FISH method, and were expressed in normalized telomere:centromere ratios (NTCRs). The autopsied individuals included 27 current smokers (CuS), 33 never-smokers (NeS), 8 ex-smokers (ExS), and 4 unknown cases. The NTCRs of CuS, NeS and ExS were 1.515, 1.372 and 1.204, respectively. The bronchial epithelial telomeres of CuS were significantly longer than those of non-current smokers (NeS+ExS). The NTCRs of the bronchial epithelium of lung cancer cases and lung cancer tissue are 1.514 and 1.385, respectively. Our findings suggest that smoking causes telomeric elongation in the bronchial epithelium. The mechanism of carcinogenesis in smoking-related carcinomas is suggested to differ from that of many other carcinomas in which genetic instability due to aging-related telomeric shortening is considered to play a role.

应用荧光原位杂交（Q-FISH）技术对72例非肺癌成人尸检标本和24例肺鳞癌手术切除标本的支气管上皮细胞端粒长度进行了测量，并以标准化端粒：着丝粒比值（NTCRs）表示。尸检的个体包括27名当前吸烟者（CuS），33名从不吸烟者（内斯），8名既往吸烟者（ExS）和4名未知病例。CuS、内斯和ExS的NTCR分别为1.515、1.372和1.204。CuS组支气管上皮细胞端粒明显长于非吸烟组（内斯+ExS）。肺癌病例支气管上皮和肺癌组织的NTCR分别为1.514和1.385。我们的研究结果表明，吸烟导致支气管上皮细胞端粒延长。吸烟相关的癌症的致癌机制，建议不同于许多其他癌症中，遗传不稳定性，由于年龄相关的端粒缩短被认为是发挥作用。

项目成果

組織切片を用いたQ-FISH法による気管支粘膜上皮のテロメア長の研究

使用组织切片的 Q-FISH 方法研究支气管粘膜上皮中的端粒长度

• 发表时间: 2013
• 作者: 相田真介;相田順子;長谷川佳代;熊坂利夫;島崎英幸;玉井誠一;田久保海誉;長村義之:
• 通讯作者: 長村義之:

Telomere length of human adult bronchial epithelium measured by tissue Q-FISH method

组织Q-FISH法测定成人支气管上皮端粒长度

• 发表时间: 2013
• 作者: Aida S;Aida J;Hasegawa K;Kumasaka T;Shimazaki H;Tamai S;Takubo K;Osamura Y
• 通讯作者: Osamura Y