Analysis for acetylation status of microtubules in epithelial mesenchymal transition induced by HGF/MET stimulation

HGF/MET刺激诱导的上皮间质转化中微管乙酰化状态分析

• 批准号: 23590431
• 负责人: MASUDA Tomoyuki
• 金额: $ 3.08万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590431/
• 关键词: 肝癌; HDAC6; アセチル化; 微小管; EMT; 分子病理; 肝細胞癌; HGF; MET; TGFβ

The aim of this study was to investigate the significance of HDAC6 in the invasion and metastasis activities of hepatocellular carcinoma (HCC). And we also examined the effect on epithelial mesenchymal trsnsition induced by HGF/MET system. HDAC6 expression was greater in all of the HCC cell lines compared to the primary cultures of hepatocytes. Knockdown of HDAC6 markedly downregulated the migration and invasion activities of all HCC cell lines (P<0.05). Overexpression of HDAC6 protein to a level higher than that in the corresponding normal hepatocytes was observed in 14 (20%) of the 70 primary HCCs, and was significantly correlated with high clinical stage, number of tumors, vascular invasion and intrahepatic metastasis (P<0.05). Downregulation of HDAC6 inhibited ERK1/2 tranlocation to the nuclesu. These results suggest that overexpression of the HDAC6 protein is involved in EMT of HCC cells.

本研究旨在探讨HDAC6在肝细胞癌侵袭转移中的意义。并检测了HGF/MET系统对上皮间质移位的影响。与原代培养的肝细胞相比，HDAC6在所有肝癌细胞系中的表达都更强。HDAC6基因敲除显著下调了所有肝癌细胞系的迁移和侵袭活性(P&lt；0.05)。在70例原发性肝癌中，有14例(20%)的HDAC6蛋白表达水平高于相应的正常肝细胞，且与高临床分期、肿瘤数目、血管侵犯和肝内转移显著相关(P&lt；0.05)。HDAC6的下调抑制了ERK1/2向核内的转位。这些结果表明，HDAC6蛋白的过表达参与了肝癌细胞的EMT。

项目成果

Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) with hemophagocytosis (HPS): successful treatment using high-dose chemotherapy (BFM-NHL & ALL-90) and autologous peripheral blood stem cell transplantation.

皮下脂膜炎样 T 细胞淋巴瘤 (SPTCL) 伴噬血细胞作用 (HPS)：使用高剂量化疗成功治疗 (BFM-NHL)

• 发表时间: 2013
• 期刊: J Clin Exp Hematop
• 影响因子: 1.5
• 作者: Sakurai E;Satoh T;Akiko YA;Maesawa C;Tsunoda K;Endo M;Akasaka T;Masuda T.
• 通讯作者: Masuda T.

Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity

• DOI: 10.3892/ijo.2012.1424
• 发表时间: 2012-07-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Ishikawa, Yuichi;Tsunoda, Kanako;Maesawa, Chihaya
• 通讯作者: Maesawa, Chihaya

Sensor and effector kinases in DNAdamage checkpoint regulate capacity for homologous recombination repair offission yeast in G2 phase.

DNA 损伤检查点中的传感器和效应激酶调节 G2 期同源重组修复裂殖酵母的能力。

• DOI: 10.1016/j.dnarep.2012.05.006
• 发表时间: 2012
• 期刊: DNA Repair (Amst).
• 作者: Yasuhira S;Saito T;Maesawa C;Masuda T.
• 通讯作者: Masuda T.

Sternoclavicular joint septic arthritis following paraspinal muscle abscess and septic lumbar spondylodiscitis with epidural abscess in a patient with diabetes: a case report.

糖尿病患者椎旁肌脓肿后胸锁关节化脓性关节炎和合并硬膜外脓肿的化脓性腰椎间盘炎：病例报告。

• 发表时间: 2012
• 期刊: BMC Emerg Med
• 影响因子: 2.5
• 作者: Shioya N;Ishibe Y;Kan S;Masuda T;Matsumoto N;Takahashi G;Makabe H;Yamada Y;Endo S.
• 通讯作者: Endo S.

Overexpression of histone deacetylase 6 contributes to accelerated migration/invasion activity of hepatocellular carcinoma cells.

组蛋白脱乙酰酶 6 的过度表达有助于加速肝细胞癌细胞的迁移/侵袭活性。

• 发表时间: 2012
• 期刊: Oncol Rep
• 影响因子: 4.2
• 作者: Oneyama C;Morii E;Okuzaki D;Takahashi Y;Ikeda J;Wakabayashi N;Akamatsu H;Tsujimoto M;Nishida T;Aozasa K;Okada M.;Kanno K
• 通讯作者: Kanno K