Lung cancer diagnosis by an mRNA assay for genes in peripheral blood mononuclear cells

通过外周血单核细胞基因 mRNA 检测诊断肺癌

• 批准号: 23590701
• 负责人: OGATA Sho
• 金额: $ 3.33万
• 依托单位: National Defense Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590701/
• 关键词: 肺癌; リボ核酸; 血中単核球分画由来リボ核酸; PCR; 血中遺伝子; 血液内遺伝子; RNA

To assess whether early lung cancer prediction might be informed by an mRNA assay for various increased genes in peripheral blood mononuclear cells (PBMNCs) and tumor tissues, we examined specimens taken from 65 carcinoma patients and 15 controls. PBMNCs and tumor-tissue specimens were taken for measurement of the mRNAs of 93 genes by TaqMan low density arrays. In the PBMNCs of the controls, 8 genes were not detected. In PBMNCs, FGF2 mRNA/GAPDH mRNA, MMP9 mRNA/GAPDH mRNA, NANOG mRNA/GAPDH mRNA, PPBP mRNA/GAPDH mRNA, S100A4 mRNA/GAPDH mRNA, STAT3 mRNA/GAPDH mRNA, and TYMP mRNA/GAPDH mRNA levels were significantly higher in the carcinoma patients than in the controls. Although the level of PPBP mRNA/GAPDH mRNA in PBMNCs appeared to correlate with that in tumor-tissue specimens, the coefficient of determination was too small (0.087). Unfortunately, measurement of mRNAs in PBMNCs may not be useful for the early diagnosis of lung carcinoma.

为了评估早期肺癌预测是否可以通过外周血单核细胞（PBMNCs）和肿瘤组织中各种增加的基因的mRNA检测来获得信息，我们检查了65例癌症患者和15例对照的标本。采用TaqMan低密度芯片检测PBMNCs和肿瘤组织中93个基因的mRNA。在对照组的PBMNCs中，有8个基因未检测到。肺癌患者外周血单个核细胞中FGF 2、MMP 9、NANOG、PPBP、S100 A4、STAT 3、TYMP mRNA/GAPDH mRNA表达水平均显著高于对照组。PBMNCs中PPBP mRNA/GAPDH mRNA的表达水平与肿瘤组织中PPBP mRNA/GAPDH mRNA的表达水平有相关性，但相关系数均为0.087。不幸的是，测量PBMNCs中的mRNA可能对肺癌的早期诊断没有帮助。

项目成果

肺癌患者における血液単核球分画中mRNAの検討

肺癌患者血液单个核细胞组分中mRNA的检测

• 发表时间: 2013
• 作者: 緒方衝;中西邦昭;島崎英幸;廣井禎之;玉井誠一
• 通讯作者: 玉井誠一