Lung cancer diagnosis by an mRNA assay for genes in peripheral blood mononuclear cells

为了评估早期肺癌预测是否可以通过外周血单核细胞（pbmnc）和肿瘤组织中各种增加基因的mRNA测定来进行，我们检查了来自65名癌症患者和15名对照患者的标本。采用TaqMan低密度阵列检测pbmnc和肿瘤组织标本中93个基因的mrna。在对照组的pbmnc中，未检测到8个基因。在pbmnc中，肿瘤患者FGF2 mRNA/GAPDH mRNA、MMP9 mRNA/GAPDH mRNA、NANOG mRNA/GAPDH mRNA、PPBP mRNA/GAPDH mRNA、S100A4 mRNA/GAPDH mRNA、STAT3 mRNA/GAPDH mRNA、TYMP mRNA/GAPDH mRNA水平均显著高于对照组。虽然pbmnc中PPBP mRNA/GAPDH mRNA水平与肿瘤组织标本中PPBP mRNA/GAPDH mRNA水平存在相关性，但其决定系数太小（0.087）。不幸的是，pbmnc中mrna的测定可能对肺癌的早期诊断没有帮助。

To assess whether early lung cancer prediction might be informed by an mRNA assay for various increased genes in peripheral blood mononuclear cells (PBMNCs) and tumor tissues, we examined specimens taken from 65 carcinoma patients and 15 controls. PBMNCs and tumor-tissue specimens were taken for measurement of the mRNAs of 93 genes by TaqMan low density arrays. In the PBMNCs of the controls, 8 genes were not detected. In PBMNCs, FGF2 mRNA/GAPDH mRNA, MMP9 mRNA/GAPDH mRNA, NANOG mRNA/GAPDH mRNA, PPBP mRNA/GAPDH mRNA, S100A4 mRNA/GAPDH mRNA, STAT3 mRNA/GAPDH mRNA, and TYMP mRNA/GAPDH mRNA levels were significantly higher in the carcinoma patients than in the controls. Although the level of PPBP mRNA/GAPDH mRNA in PBMNCs appeared to correlate with that in tumor-tissue specimens, the coefficient of determination was too small (0.087). Unfortunately, measurement of mRNAs in PBMNCs may not be useful for the early diagnosis of lung carcinoma.