The effects of itch/scratching on allergic dermatitis.

我们使用小鼠皮炎模型检查瘙痒/抓伤与过敏性皮炎的关系。用半抗原和BALB/c小鼠检测小鼠接触性超敏反应后12和24小时的基因转录。我们发现在12和24小时分别有127个和8个基因发生差异转录。此外，中性粒细胞趋化剂mip2 - α被确定为皮肤炎症的初始反应基因。在缺乏mip2 - α受体CXCR2的小鼠中，预计接触超敏反应会受损，但有趣的是，敲除小鼠和对照组之间的反应没有变化。包括局部免疫组织学在内的进一步调查正在进行中。在另一种缺乏组织蛋白酶E敲除小鼠的模型小鼠中，由于其后腿的恶性运动，我们无法正确评估搔抓。

We examined association of itch/scratching and allergic dermatitis using mouse models of dermatitis. Gene transcription at 12 and 24 hours after challenge in murine contact hypersensitivity reaction using hapten and BALB/c mice were examined. We found that A total of 127 and 8 genes were differentially transcripted at 12 and 24 hours, respectively. Furthermore, the neutrophil chemoattractant MIP2-alpha was identified as initial response gene in the skin inflammation. Impaired contact hypersensitivity reaction was expected in mice lacking CXCR2, the receptor for MIP2-alpha, but interestingly, there was no change in the reaction between the knockout mice and controls. Further investigation including local immunohistology is ongoing. We could not properly assessed the scratching in another model mice lacking cathepsin E knockout mice, due to its vicious movement of the hind legs of the mice.