Detection of novel actions of kampo medicines for lipid metabolism by lipidomic analysis

通过脂质组学分析检测汉方药物对脂质代谢的新作用

• 批准号: 23590873
• 负责人: SHIRO Watanabe
• 金额: $ 3.24万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590873/
• 关键词: 胆汁酸; トリグリセリド; 一価不飽和脂肪酸; ステアリン酸CoA不飽和化酵素1; ステアリン酸CoA不飽和化酵素１; コレステロールエステル; トランスアミナーゼ; コール酸; 肝傷害; エイコサペンタエン酸; リポプロテインリパーゼ

The administration of Boiogito (BOT) was shown to ameliorate liver injury in the mice fed a cholate (CA)-supplemenetd diet. This effect was associated with a partial restoration of the reduction in liver triglyceride (TG) content. The levels of gene and protein of stearoyl-CoA desaturase-1 (SCD1) were lowered in the mice fed the CA-supplemented diet, which were elevated by BOT administration. These results suggest that the amelioration of CA-induced liver injury by BOT administration may be due to the restoration of reduced synthesis of liver TG through the facilitation of desaturation of saturated fatty acids, which is required in the efficient TG synthesis in liver. Alternatively, the reduction in liver TG levels might be a consequence of the amelioration of CA-induced liver injury by BOT administration. Thus, our lipidomic analysis revealed a novel mechanism involved in the modulation of lipid metabolism by a kampo medicine during bile acid-induced steatohepatitis in the mice.

Boiogito （BOT）被证明可以改善饲喂巧克力（CA）补充饮食的小鼠的肝损伤。这种效果与肝脏甘油三酯（TG）含量减少的部分恢复有关。脂酰辅酶a去饱和酶-1 （SCD1）基因和蛋白水平在添加ca的小鼠中降低，而在添加BOT的小鼠中升高。这些结果表明，BOT对ca诱导的肝损伤的改善可能是由于通过促进饱和脂肪酸的去饱和来恢复肝脏中减少的TG合成，而饱和脂肪酸是肝脏中有效合成TG所必需的。另外，肝脏TG水平的降低可能是通过给药BOT改善ca诱导的肝损伤的结果。因此，我们的脂质组学分析揭示了一种新的机制，涉及在胆汁酸诱导的小鼠脂肪性肝炎期间由坎坡药物调节脂质代谢。

项目成果

Evaluation of the Quality of Chinese and Vietnamese Cassia Using LC-MS and Multivariate Analysis

• DOI: 10.1177/1934578x1300800118
• 发表时间: 2013-01
• 期刊: Natural Product Communications
• 影响因子: 1.8
• 作者: Ken Tanaka;Feng Li;Y. Tezuka;Shiro Watanabe;N. Kawahara;H. Kida

Eicosapentaenoic acid attenuates hepatic accumulation of cholesterol esters but aggravates liver injury and inflammation in mice fed a cholate-supplemented high-fat diet

• DOI: 10.2131/jts.38.379
• 发表时间: 2013-06-01
• 期刊: JOURNAL OF TOXICOLOGICAL SCIENCES
• 影响因子: 2
• 作者: Watanabe, Shiro;Tsuneyama, Koichi
• 通讯作者: Tsuneyama, Koichi

Pig bile reduces hepatic triglyceride content in mice

猪胆汁降低小鼠肝脏甘油三酯含量

• 发表时间: 2013
• 期刊: J. Trad. Med.
• 作者: Watanabe S.;Fujita K.
• 通讯作者: Fujita K.

牛胆は一価不飽和脂肪酸含有コレステロールエステルの優位な蓄積を伴う脂肪肝を誘導する

牛胆诱发脂肪肝，主要积聚含单不饱和脂肪酸的胆固醇酯

• 发表时间: 2011
• 作者: Tawada M;Hayashi S(他3名);渡辺志朗;渡辺志朗,常山幸一
• 通讯作者: 渡辺志朗,常山幸一

Eicosapentenoic acid supplementation attenuates hepatic accumulation of cholesterol esters but aggravates liver injury in mice fed a fat- and sodium cholate-supplemented diet in mice

补充二十碳五烯酸可减少胆固醇酯在肝脏中的积累，但会加重饲喂补充脂肪和胆酸钠饮食的小鼠的肝损伤

• 发表时间: 2012
• 作者: Ohta Y;Hayashi S 他;竹屋泰;Shiro Watanabe
• 通讯作者: Shiro Watanabe