COX-2 gene promoter methylation in patients infected with Helicobacter pylori.

幽门螺杆菌感染患者COX-2基因启动子甲基化。

• 批准号: 23590928
• 负责人: YASUDA Hiroshi
• 金额: $ 3.24万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590928/
• 关键词: 胃潰瘍; COX-2; DNAメチル化; COX2; ピロリ菌; スナネズミ; 消化性潰瘍; 胃癌

[Aim] COX2 plays a critical role in peptic ulcer development. We evaluated COX2 methylation levels in the gastric mucosa. [Methods] DNA was extracted from endoscopic biopsy materials. The COX2 methylation levels were measured quantitatively by pyrosequencing. COX2 mRNA expression was measured using real-time PCR. [Results] COX2 methylation levels were significantly higher in H.pylori (HP)-positive cases than in HP-negative cases. COX2 methylation levels in patients in whom HP was successfully eradicated were significantly lower than that in HP-positive cases. COX2 mRNA expression was not observed in Kato III cells, despite the addition of the protein kinase C stimulator (PDBu). COX2 expression was observed after the addition of the demethylating agent and was enhanced by PDBu.[Conclusion] HP infection caused a significant increase in the COX2 methylation levels in the gastric mucosa. In addition to transcriptional regulation, COX2 expression is regulated through epigenetic mechanisms.

[目的]COX2在消化性溃疡的发生发展中起关键作用。我们评估了胃粘膜中COX2甲基化水平。[方法]从内窥镜活检材料中提取DNA。焦磷酸测序定量检测COX2甲基化水平。实时定量聚合酶链式反应检测COX2基因的表达。[结果]幽门螺杆菌(Hp)阳性组COX2甲基化水平显著高于Hp阴性组。根除Hp成功的患者COX2甲基化水平显著低于Hp阳性患者。尽管加入了蛋白激酶C刺激物(PDBu)，但在Kato III细胞中未观察到COX2mRNA的表达。加入脱甲基剂后可观察到COX2的表达，并被PDBu增强。[结论]幽门螺杆菌感染引起胃粘膜COX2甲基化水平显著升高。除了转录调控外，COX2的表达还通过表观遗传机制进行调控。

项目成果

Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer.

微卫星缺乏结直肠癌中MLL3帧速率突变的频繁改变。

• DOI: 10.1371/journal.pone.0023320
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Watanabe Y;Castoro RJ;Kim HS;North B;Oikawa R;Hiraishi T;Ahmed SS;Chung W;Cho MY;Toyota M;Itoh F;Estecio MR;Shen L;Jelinek J;Issa JP
• 通讯作者: Issa JP

COX2 hypermethylation in HP-positive GU patients.

HP 阳性 GU 患者中 COX2 高甲基化。

• 发表时间: 2011
• 作者: Yasuda H;Watanabe Y;Itoh F
• 通讯作者: Itoh F

A New Detection Method for Integration of Hepatitis B virus DNA in PLC/PRF/5 Cells

PLC/PRF/5细胞中乙型肝炎病毒DNA整合的新检测方法

• 发表时间: 2012
• 期刊: Journal of St.Marianna University
• 作者: Hiraishi T;Watanabe Y;Oikawa R;Hara M;Kiyokawa H;Yoshida Y;Yamada N;Okuse C;Suzuki M;Yotsuyanagi H;Itoh F
• 通讯作者: Itoh F

プロトンポンプ阻害剤と抗血小板薬クロピドグレル, プラスグレルの最近の話題・消化器内科の立場から(PPI併用慎重派)

质子泵抑制剂和抗血小板药物氯吡格雷和普拉格雷近期话题——从胃肠病学角度（与PPI联用需谨慎）

• 发表时间: 2011
• 期刊: 臨床薬理
• 作者: Ishigoooka S;Nomoto M;Obinata N;Oishi Y;Sato Y;Nakatsu S;Suzuki M;Ikeda Y;Maehata T;Kimura T;Watanabe Y;Nakajima T;Yamano HO;Yasuda H;Itoh F.;安田宏
• 通讯作者: 安田宏

門脇孝・永井良三総編集 「カラー版 内科学」

门胁隆、永井良三主编《内科彩色版》

• 发表时间: 2012
• 作者: Yasuda H;Watanabe Y;Itoh F;安田宏（分担執筆）;安田宏 （分担執筆）
• 通讯作者: 安田宏 （分担執筆）